Dataset Information


Escherichia coli str. K-12 substr. MG1655

ABSTRACT: Identification of RNAs bound to MS2 aptamer using MS2-affinity purification coupled with RNA sequencing (MAPS)

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655  



Dataset's files

Action DRS
SRR1953000_1.fastq.gz Fastqsanger.gz
SRR1953000_2.fastq.gz Fastqsanger.gz
SRR1952999_1.fastq.gz Fastqsanger.gz
SRR1952999_2.fastq.gz Fastqsanger.gz
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DsrA regulatory RNA represses both hns and rbsD mRNAs through distinct mechanisms in Escherichia coli.

Lalaouna David D   Morissette Audrey A   Carrier Marie-Claude MC   Massé Eric E  

Molecular microbiology 20150904 2

The 87 nucleotide long DsrA sRNA has been mostly studied for its translational activation of the transcriptional regulator RpoS. However, it also represses hns mRNA, which encodes H-NS, a major regulator that affects expression of nearly 5% of Escherichia coli genes. A speculative model previously suggested that DsrA would block hns mRNA translation by binding simultaneously to start and stop codon regions of hns mRNA (coaxial model). Here, we show that DsrA efficiently blocked translation of hn  ...[more]

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