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Mus musculus

ABSTRACT: Next Generation Sequencing Analysis of Wild Type and Rfx2-/- Testicular Transcriptomes [RNA-Seq P30]

OTHER RELATED OMICS DATASETS IN: E-GEOD-68284E-GEOD-68283

PROVIDER: PRJNA282463 | ENA |

REPOSITORIES: ENA

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Project description:Next Generation Sequencing Analysis of Wild Type and Rfx2-/- Testicular Transcriptomes

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Transcription Factor RFX2 Is a Key Regulator of Mouse Spermiogenesis

Project description:To identify RFX2-dependent genes during spermatogenesis, We chose 24-day-old wild-type and mutant testes samples. Spermiogenesis in mutants was normal before this time point while massive round spermatids detached from the tubules thereafter. RNAs were isolated from the total testicular cells.

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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and L3mbtl2 cKO Testicular Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and L3mbtl2 cKO Testicular Transcriptomes

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Next Generation Sequencing Analysis of Wild Type and Rfx2-/- Testicular Transcriptomes at post natal day 21 [RNA-Seq P21]

Project description:Purpose: This study was carried out to determine the consequences of the Rfx2-/- genotype on spermatogenesis in the mouse Methods: RNA was extracted from decapsulated testes of 21 day old mixed background mice of either genotype. Deep sequencing was used to determine quantitative expression of the genomes from independent replicates of each genotype Results: RNA-Seq analysis identified some 105 genes that are down regulated at least 2-fold in Rfx2-/- testes, with ~50 being reduced at least 10-fold Conclusion: Spermatogenesis undergoes complete arrest just prior to the end of the round spermatid period of sperm development in mutant mice. Sequencing results showed that approximately 105 genes were downregulated 2 fold or more in the testes of mutant mice. Comparison of similar studies of targeted mutations in genes for other transcription factor demonstrate that Rfx2 has a large and nearly unique set of genes that depend on it directly or indirectly. A large number of downregulated genes are identified with cilia function. Testicular mRNA profiles were determined by deep sequencing using testes from 5 independent wild type and 6 independent Rfx2-/- mice

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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and FXRalpha-/- testicular Transcriptomes with Environmental Exposure

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and FXRalpha-/- testicular Transcriptomes with Environmental Exposure

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