Project description:The atmosphere CO2 concentration keeps increasing every year. Use the Affymetrix poplar gene chip to confirm the expression changes in key genes in the triploid white poplar due to the influence of elevated CO2 concentrations. We used microarrays to detail the global programme of gene expression under normal and elevated CO2 concentrations. Gene expression of triploid white poplar ((P. tomentosa Ã? P. bolleanaï¼?Ã? P. tomentosa) leaves were investigated by using the Affymetrix poplar genome gene chip, after grown in controlled environment chambers under three different CO2 concentrations. Poplar leaves were subjected to normal CO2 concentrations (T0) and elevated CO2 concentrations (T1, 550 ppm and T2, 720 ppm) treatments three months.
Project description:We take the two year old plant for sampling.Use the Affymetrix poplar gene chip to elucidate the gene functions and mechanisms in Populus tomentosa shoot apex and mature xylem. We used microarrays to detail the global programme of gene expression in shoot apex and mature xylem. Populus tomentosa shoot apex and mature xylem were taken for RNA extraction and hybridization on Affymetrix microarrays.CB2009304-C and CB2009304-D from shoot apex, CB2009304-G and CB2009304-H from mature xylem.
Project description:We take the two year old plant for sampling.Use the Affymetrix poplar gene chip to elucidate the gene functions and mechanisms in Populus tomentosa shoot apex and mature xylem. We used microarrays to detail the global programme of gene expression in shoot apex and mature xylem.
Project description:We take the two year old plant for sampling. Use the Affymetrix poplar gene chip to elucidate the gene functions and mechanisms in Populus tomentosa newly formed developing xylem and lignified xylem. We used microarrays to detail the global programme of gene expression in newly formed developing xylem and lignified xylem. Populus tomentosa newly formed developing xylem and lignified xylem were taken for RNA extraction and hybridization on Affymetrix microarrays. CB2009304-A and CB2009304-B from newly formed developing xylem, CB2009304-G and CB2009304-H from lignified xylem.
Project description:We take the two year old plant for sampling. Use the Affymetrix poplar gene chip to elucidate the gene functions and mechanisms in Populus tomentosa newly formed developing xylem and lignified xylem. We used microarrays to detail the global programme of gene expression in newly formed developing xylem and lignified xylem.
Project description:We sequenced mRNA from the control and heat treatments leaves of Populus tomentosa using the Illumina HiSeq4000 platform to generate the transcriptome dynamics that may serve as a gene expression profile blueprint for different response patterns under control and heat stress in Populus tomentosa.
Project description:Poplar GeneChip was employed to detect genes expressed during the whole floral developmental process, in order to improve understanding of poplar flower development, since current knowledge on flower development was mainly from model plant Arabidopsis. Male and female floral buds of Populus tomentosa were selected at successive stages of the whole development process for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain genes contributed to floral development, but not the dynamic expression changes. To that end, equal amount of floral buds RNA per gender from different stages were mixed for the detection of expressed genes.
Project description:Global gene expression pattern of different poplar tissue types were determined using a Nimblegen microarray based on JGI v1.1 gene models. All tissue except reproductive tissue were obtained from the same clone used for the poplar genome sequencing project (Populus trichocarpa Nisqually-1). Reproductive tissue were from wild Populus trichocarpa trees.