Project description:To investigate the effects of rdr2 and rdr6 mutations upon the strucutred component of the Physcomitrella patens transcriptome, dsRNAseq performed. The accumulation of dsRNAseq reads within known small RNA (microRNA and siRNA) loci was quantified and compared between wild-type and the two mutants. Total RNA samples were obtained from wild-type, rdr2, and rdr6, and subject to dsRNAseq via the protocol described by Zheng et al. (2010) (PMID: 20941385)
Project description:To investigate the effects of dcl3,dcl4,mDCL,rdr2,rdr6,nrpd1b, and nrpd1c mutations upon small RNAs in Physcomitrella patens , small RNA-seq was performed. Total RNA samples were obtained from the indicated mutants and subject to small RNA-seq using the Illumina TruSeq Small RNA protocol, and analyzed on a HiSeq2500 instrument
Project description:To investigate the effects of rdr2 and rdr6 mutations upon small RNAs in Physcomitrella patens , small RNA-seq was performed. Total RNA samples were obtained from wild-type, rdr2, and rdr6, and subject to small RNA-seq via the protocol described by Zheng et al. (2010) (PMID: 20941385)
Project description:High-throughput sequencing of endogenous small RNAs from the moss Physcomitrella patens. This dataset encompasses microRNAs and other small RNAs of ~20-24 nucleotides expressed in the moss P. patens. SAMPLES UPDATED JULY 9, 2007 TO INCLUDE DATA ON SEQUENCED SMALL RNAS THAT DO NOT MATCH THE P. PATENS GENOME Keywords: High throughput small RNA sequencing Overall design: Small RNA cDNA libraries derived from polyacrylamide gel electrophoresis (PAGE) fractionated total RNA, adapter ligation, reverse transcription, and pyrosequencing was used for microRNA and endogenous siRNA discovery in wild-type tissues of the moss Physcomitrella patens. See Axtell et al. (2006) A two-hit trigger for siRNA biogenesis in plants. Cell 127: 565-577. for details.
Project description:Temperature reduction is a common environmental stress for plants. Land plants need to cope with cold stress on the basis of complex transcriptional and metabolic changes. The transcriptional responses and signaling networks that contribute to cold acclimation of seed plants have been analyzed previously. Here, we present the whole-genome transcriptomic cold stress response of the model moss species Physcomitrella patens as the representative of an early diverged lineage of haploid-dominant and poikilohydric land plants On the basis of time-series microarray experiments we characterized transcriptomic changes related to early stress signaling and the initiation of cold-acclimatory mechanisms, and as secondary effects, of dehydration and oxidative stress.