Project description:Background: Pantoea ananatis LMG 2665T synthesizes and utilizes acyl homoserine lactones (AHLs) for signaling. In this strain, short chain AHLs (C4 to C8) are produced by the EanI/R quorum sensing (QS) system that is involved in pathogenicity and biofilm formation. The complete set of genes regulated by the EanI/R system in P. ananatis LMG 2665T is still not fully known. In the present study, RNA-seq was used to analyze the transcriptome profiles controlled by the EanI/R system in this strain by comparing the wild type strain and its QS mutant 2665T ean∆I/R during lag and log stages. The RNA seq data was validated by RT qPCR. Results: The results showed that the EanI/R regulon in P. ananatis LMG 2665T comprised 144 genes, constituting 3.3% of the whole transcriptome under the experimental conditions in this study. The majority of genes regulated by the EanI/R system included genes for flagella assembly, bacterial chemotaxis, pyruvate metabolism, two component system, metabolic pathways, microbial metabolism and biosynthesis of secondary metabolites. Conclusions: This is the first study to identify the EanI/R QS regulon in P. ananatis LMG 2665T. Functional analysis of genes regulated the EanI/R system in LMG 2665T could help unveil genes that play a vital role in pathogenesis and survival strategies of this pathogen.

2018-10-31 | GSE87207 | GEO

Campylobacter ureolyticus strain:LMG 6451

Project description:Campylobacter ureolyticus strain:LMG 6451 Genome sequencing

| PRJNA633574 | ENA

Campylobacter blaseri strain:LMG 30333

Project description:Campylobacter blaseri strain:LMG 30333 Genome sequencing and assembly

| PRJNA521388 | ENA

Campylobacter ornithocola strain:LMG 29815

Project description:Campylobacter ornithocola strain:LMG 29815 Genome sequencing and assembly

| PRJNA521390 | ENA

Campylobacter corcagiensis strain:LMG 27932

Project description:Campylobacter corcagiensis strain:LMG 27932 Genome sequencing and assembly

| PRJNA521387 | ENA

microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach

Project description:microRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at post-transcriptional level and play essential roles in fundamental biological processes such as development and metabolism. Here, we perform a comprehensive analysis of miRNAs in the zoonotic parasite E. canadensis G7, one of the causative agents of the neglected disease cystic echinococcosis. Small RNA libraries from protoscoleces and cyst walls of E. canadensis G7 and protoscoleces of E. granulosus sensu stricto G1 were sequenced using Illumina technology. As a result, we found transcriptional evidence of 37 miRNAs thus expanding the miRNA repertoire of E. canadensis G7. Differential expression analysis showed significant regulated miRNAs between life cycle stages of E. canadensis G7. We confirmed the remarkable loss of conserved miRNA families in E. canadensis, reflecting their low morphological complexity and high adaptation to parasitism. This study will provide valuable information for better understanding the complex biology of this parasite and could help to find new potential targets for therapy and/or diagnosis.

2015-02-09 | GSE64705 | GEO

Campylobacter coli LMG 23336

Project description:Campylobacter coli LMG 23336 genome sequencing

| PRJNA52743 | ENA