Genomics

Dataset Information

1

Mycoplasma mycoides subsp. capri str. GM12 isolate:YCpMmyc1.1-deltaglf


ABSTRACT: Mutant Mycoplasma mycoides subsp. capri genome sequencing and assembly

PROVIDER: PRJNA285728 | ENA |

REPOSITORIES: ENA

Dataset's files

Source:
Action DRS
SRR2048552_subreads.fastq.gz Fastqsanger.gz
GCA_001267815.1_sequence_report.txt Txt
Items per page:
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Publications


We recently reported the chemical synthesis, assembly, and cloning of a bacterial genome in yeast. To produce a synthetic cell, the genome must be transferred from yeast to a receptive cytoplasm. Here we describe methods to accomplish this. We cloned a Mycoplasma mycoides genome as a yeast centromeric plasmid and then transplanted it into Mycoplasma capricolum to produce a viable M. mycoides cell. While in yeast, the genome was altered by using yeast genetic systems and then transplanted to prod  ...[more]

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