Project description:Here, de novo transcriptome assemblies for leaf and flower tissues of Forsythias were performed, resulting in 81913 unigenes of F. suspensa, 88491 unigenes of F. viridissima and 69458 unigenes of F. koreana (F. viridissima var. koreana). Classification of the annotated unigenes by gene ontology terms and KEGG pathways was used to explore transcriptomic differences among the Forsythias. Orthogroup was introduced to compare expression levels of unigenes in tissues from different species, which unveiled that three leaf tissues of Fosythias were closely correlated based on expression values of orthologous unigenes. Showing high expression mainly in leaves of F. viridissima and F. koreana, candidate homologs for genes involved in the biosynthetic pathway of lignans and phenylethanoid glycosides were determined in these transcriptome assemblies.
Project description:Here, de novo transcriptome assemblies for leaf and flower tissues of Forsythias were performed, resulting in 81913 unigenes of F. suspensa, 88491 unigenes of F. viridissima and 69458 unigenes of F. koreana (F. viridissima var. koreana). Classification of the annotated unigenes by gene ontology terms and KEGG pathways was used to explore transcriptomic differences among the Forsythias. Orthogroup was introduced to compare expression levels of unigenes in tissues from different species, which unveiled that three leaf tissues of Fosythias were closely correlated based on expression values of orthologous unigenes. Showing high expression mainly in leaves of F. viridissima and F. koreana, candidate homologs for genes involved in the biosynthetic pathway of lignans and phenylethanoid glycosides were determined in these transcriptome assemblies.
Project description:Here, de novo transcriptome assemblies for leaf and flower tissues of Forsythias were performed, resulting in 81913 unigenes of F. suspensa, 88491 unigenes of F. viridissima and 69458 unigenes of F. koreana (F. viridissima var. koreana). Classification of the annotated unigenes by gene ontology terms and KEGG pathways was used to explore transcriptomic differences among the Forsythias. Orthogroup was introduced to compare expression levels of unigenes in tissues from different species, which unveiled that three leaf tissues of Fosythias were closely correlated based on expression values of orthologous unigenes. Showing high expression mainly in leaves of F. viridissima and F. koreana, candidate homologs for genes involved in the biosynthetic pathway of lignans and phenylethanoid glycosides were determined in these transcriptome assemblies.
Project description:Comparative transcriptome analyses of three medicinal Forsythia species and prediction of candidate genes involved in secondary metabolisms [Forsythia viridissima var. koreana]
Project description:The conservation of the endangered Korean fir, Abies koreana, is of critical ecological importance. In our previous study, a yeast-like fungus identified as Aureobasidium pullulans AK10, was isolated and shown to enhance drought tolerance in A. koreana seedlings. In this study, the effectiveness of A. pullulans AK10 treatment in enhancing drought tolerance in A. koreana was confirmed. Furthermore, using transcriptome analysis, we compared A. koreana seedlings treated with A. pullulans AK10 to untreated controls under drought conditions to elucidate the molecular responses involved in increased drought tolerance.
