Project description:Transcriptional profiling of Bacillus subtilis to lethal heat stress by systematic sampling of bacterial cell suspensions exposed to gradually increasing temperatures. Keywords: Stress response Bacterial cell suspensions exposed to increasing temperatures ranging from 30-62ºC (Cy5 channel) versus 30 degrees C reference (Cy 3 channel), by transfer of the bacterial cell suspension from a 30ºC to a 65ºC water bath; sampling and quenching at 30, 40, 50, 57, 58, 59, 60 and 62ºC; total time to reach 62ºC is ~12 minutes . Biological replicates: 2, independently grown cultures. One replicate per array. Strains B subtilis 168 1A1; B subtilis A163 (food product isolate); B. subtilis MC85 (food product isolate).
Project description:Legionella pneumophila Philadelphia-1 strain was grown to stationary phase in AYE broth and starved in freshwater for 2 hours and RNA was harvested with or without sublethal heat shock via immersion in a 55 degree C hot water bath for 5 minutes
Project description:We have performed quantitative phosphoproteomic analysis on jurkat cells. Phosphorylation change was compared between jurkat cells incubated on ice and those incubated in 37 degree water bath. And the combination of cold induced and OKT3/4 antibody induced signaling was compared.
Project description:These experiments were designed to study the effect of heat shock and cold shock on expression profiles of a hyperthermophilic archaeon after a twenty-minute temperature shock. When cells entered the middle of the exponential growth phase at the optimal growth temperature of 85 C, they were transferred from an 85 C water bath to a 95 C water bath or a 65 C water bath. Two biological replicates were obtained under both heat shock and cold shock conditions. For each biological replicate, we performed a total of six technical replicates consisting of three pairs of flip-dye experiments. As for the reference sample, a pooled population of cDNA from three biological replicates consisting of cells growing in the mid-exponential phase at 85 C was used. All hybridizations were performed against this reference
Project description:Expression profile of wild-type S. ovata and methanol-adapted strain grown autotrophically with H2 as the source of electron. Illumina RNA-Seq of total RNA extracted from a strain of S. ovata evolved by adaptive laboratory evolution to grow faster with methanol 2% as the sole susbtrate and from the wild type. The adapted strain was transfer 18 times on methanol 2%. Total RNA was extracted from both strains growing with H2/CO2.
