Project description:To assess the performance of our custom-designed pan-genome microarray and characterize the differences in gene content between JP2 and non-JP2 genotypes of A. actinomycetemcomitans. Comparative genomic hybridization reactions were carried out to assess the performance of our pan-genome microarray by using genomic DNA of 11 previously sequenced Aggregatibacter actinomycetemcomitans strains (I23C, SCC1398, ANH9381, HK1651, D7S-1, D11S-1, I63B, SCC393, D18P-1, SCC2302, and D17P-2). The microarray was subsequently used for comparative genomic hybridization of 6 strains of JP2 (S067, A26, G111-1, G121-2, D41S-1, and HK1651) and 6 strains non-JP2 (I23C, SCC1398, ANH9381, ATCC29524, 194, and G104-2) genotypes of Aggregatibacter actinomycetemcomitans. Two biological duplicates were carried out for each strain.
Project description:To analyze the gain and lost of genes in Aggregatibacter actinomycetemcomitans during short-term persistent infection in the same host The microarray was used to compare gene contents of 4 different pairs of Aggregatibacter actinomycetemcomitans strains (SCC393 vs A160, SCC1398 vs SCC4092, SCC2302 vs AAS4a, and S23A vs I23C), where each pair was isolated from an individual over time. Two biological duplicates were carried out for each strain.
Project description:To analyze the gain and lost of genes in Aggregatibacter actinomycetemcomitans during short-term persistent infection in the same host