Project description:We report a study conducted to investigate the variation on gene expression of the pathogenic fungus Aspergillus fumigatus upon co-cultivation with the pathogenic bacterium Pseudomonas aeruginosa. The study was conducted by investigating the gene expression variation at different time points (45, 90 and 180 minutes after co-incubation). As control, we used data obtained by cultivating the fungus either without bacteria, or with heat-inactivated Pseudomonas. Overall design: Examination of Aspergillus fumigatus co-cultivated with Pseudomonas aeruginosa.
Project description:Carolacton is a novel biofilm inhibitor that kills biofilm cells of Streptococcus mutans and inhibits growth of the clinically relevant and human pathogenic bacterium Streptococcus pneumoniae TIGR4 in nanomolar concentrations. Interestingly, Carolacton also inhibits Escherichia coli with a defective outer membrane protein TolC . The cellular target of Carolacton is still unknown. Here, we adressed the differential transcription of cellular RNAs when E.coli TolC was grown in the presence of Carolacton. This was done to identify transcriptional regulatory networks that are directly affected by treatment of the strain with Carolacton. In order to gain insights into the primary transcriptional response, early time-points were chosen for sampling, which should not reflect secondary responses (e.g. due to differences in growth phase, nutrient depletion etc.). Overall design: For each of the four examined time-points (0, 5, 15, 30), two biological replicates of Carolacton-treated and untreated samples were collected.