Project description:In previous research, we have discovered a phenomenon of inducible antagonistic activity in strains of the order Bacillales in which, large inhibition zones were produced against different pathogens when growing in the presence of the chemical compound triphenyl tetrazolium chloride (TTC), where Bacillus subtilis NCIB3610, Bacillus cereus EA-CB1047 and B. tequilensis EA-CB0015 how high activity against the phytopathogen Ralstonia solanacearum. The exact structure of these compounds has not been so far elucidated; therefore, this project aims to clarify whether the changes in the metabolic profiles of Bacillus sp. correspond to biotransformation reactions of TTC or might be products of any additional biosynthetic process not yet revealed, using molecular networks approaches.
Project description:The aim of the experiments was to determine the regulon of the Bacillus subtilis alternative sigma factor SigI. Biological relevance: To expand our knowledge about Bacillus subtilis transcriptional network under unfavorable conditions. Experimental workflow overview: Bacillus subtilis 168 trp+ (BaSysBio) was used as the genetic background. (i) sigI-rsgI knock-out, (ii) rsgI knock-out, and (iii) wr strains were cultured in LB medium to mid-exponential phase at 37°C and 52°C. Total RNA was isolated from 3 ml of the culture. rRNA was depleted from the samples with RiboMinus; subsequently RNA-seq libraries were prepared (Illumina compatible NEXTflex Rapid Directional RNA-Seq Kit, Bioo Scientific) and sequenced at the EMBL GeneCore facility. The experiment was performed in three independent replicates.
Project description:Identification of the specific WalR (YycF) binding regions on the B. subtilis chromosome during exponential and phosphate starvation growth phases. The data serves to extend the WalRK regulon in Bacillus subtilis and its role in cell wall metabolism, as well as implying a role in several other cellular processes.
