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M1A marks translation initiation sites in human and mouse messenger RNA [Sequencing]

ABSTRACT: m1A marks translation initiation sites in human and mouse messenger RNA [Sequencing]

PROVIDER: PRJNA288805 | ENA |

REPOSITORIES: ENA

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m1A marks translation initiation sites in human and mouse messenger RNA [Sequencing]

2016-02-10 | E-GEOD-70485 | biostudies-arrayexpress

m1A marks translation initiation sites in human and mouse messenger RNA [microarray]

Project description:We developed a novel approach, m1A-seq, for high-resolution mapping of the transcriptome-wide m1A landscape, based on antibody-mediated capture followed by massively parallel sequencing. Using this method we performed immunodepletion of methylated transcipts to assess the average methylation level Immunodepletion of m1A modified transcripts in human compared to the input, allows for estimation of the fraction of methylated transcripts. We compared the expression levels of sup (immunodepleted) and input methylated transcripts to deduce the fraction of m1A methylation.

2016-02-12 | E-GEOD-76057 | biostudies-arrayexpress

m1A marks translation initiation sites in human and mouse messenger RNA [Sequencing]

2016-02-10 | GSE70485 | GEO

m1A marks translation initiation sites in human and mouse messenger RNA [microarray]

2016-02-12 | GSE76057 | GEO

m1A marks translation initiation sites in human and mouse messenger RNA

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-02-12 | E-GEOD-76058 | biostudies-arrayexpress

m1A marks translation initiation sites in human and mouse messenger RNA

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-02-12 | GSE76058 | GEO

m1A-Seq analyses of trophoblast upon hypoxia or normoxia

Project description:N1-methyladenosine (m1A) is one of messenger RNA modification in eukaryotes, but the potential roles of m1A methylated mRNA in trophoblast upon hypoxia remain elusive.

2020-03-24 | GSE135403 | GEO

Project description:m1A marks translation initiation sites in human and mouse messenger RNA

| PRJNA306096 | ENA

Homo sapiens

Project description:m1A marks translation initiation sites in human and mouse messenger RNA [microarray]

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Chemical manipulation of m1A mediates its detection in human tRNA

Project description:N1-methyl adenosine (m1A) is a wide-spread RNA modification present in tRNA, rRNA and mRNA. m1A modification sites in tRNAs are evolutionary conserved and its formation on tRNA is catalyzed by methyltransferase TRMT61A and TRMT6 complex. m1A promotes translation initiation and elongation. Due to its positive charge under physiological conditions, m1A can notably modulate RNA structure. It also blocks Watson-Crick base pairing and causes mutation and truncation during reverse transcription. Several misincorporation-based high throughput sequencing methods have been developed to sequence m1A. In this study, we introduce a reduction-based m1A sequencing (red-m1A-seq). We report that NaBH4 reduction of m1A can improve the mutation and readthrough rates using commercially available RT enzymes to give better positive signature, while alkaline-catalyzed Dimroth rearrangement can efficiently convert m1A to m6A to provide good controls, allowing the detection of m1A with higher sensitivity and accuracy. We applied red‑m1A-seq to sequence human small RNA and we not only detected all the previously reported tRNA m1A sites, but also new m1A sites in mt-tRNAAsn-ATT and 5.8S rRNA.

2024-04-24 | GSE253657 | GEO

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