Dataset Information


Trypanosoma brucei

ABSTRACT: Antisense Transcripts Impede Uridylation-induced Exonucleolytic Degradation to Generate Guide RNAs

ORGANISM(S): Trypanosoma brucei  



altmetric image


Antisense Transcripts Delimit Exonucleolytic Activity of the Mitochondrial 3' Processome to Generate Guide RNAs.

Suematsu Takuma T   Zhang Liye L   Aphasizheva Inna I   Monti Stefano S   Huang Lan L   Wang Qi Q   Costello Catherine E CE   Aphasizhev Ruslan R  

Molecular cell 20160128 3

Small, noncoding RNA biogenesis typically involves cleavage of structured precursor by RNase III-like endonucleases. However, guide RNAs (gRNAs) that direct U-insertion/deletion mRNA editing in mitochondria of trypanosomes maintain 5' triphosphate characteristic of the transcription initiation and possess a U-tail indicative of 3' processing and uridylation. Here, we identified a protein complex composed of RET1 TUTase, DSS1 3'-5' exonuclease, and three additional subunits. This complex, termed  ...[more]

Similar Datasets

2016-02-08 | E-GEOD-71292 | ArrayExpress
2017-05-18 | MODEL1511170001 | BioModels
2017-05-18 | MODEL1511170002 | BioModels
2017-05-18 | MODEL1511170000 | BioModels
| GSE81537 | GEO
| GSE111152 | GEO
| GSE46160 | GEO
2010-02-23 | E-GEOD-19734 | ArrayExpress
2010-02-23 | GSE19734 | GEO
2014-04-21 | E-GEOD-38636 | ArrayExpress