ABSTRACT: Co-culture confrontation assays of Klebsiella pneumoniae vs. other multi-drug resistance organisms or vs. human dermal fibroblasts. transcriptome
Project description:Co-culture confrontation assays of Acinetobacter baumannii vs. other multi-drug resistance organisms or vs. human dermal fibroblasts. transcriptome
| PRJNA292770 | ENA
Project description:Co-culture confrontation assays of Acinetobacter baumannii vs. other multi-drug resistance organisms or vs. human dermal fibroblasts. Transcriptome
Project description:Co-culture confrontation assays of Enterobacter cloacae vs. other multi-drug resistance organisms or vs. human dermal fibroblasts. transcriptome
Project description:Co-culture confrontation assays of commercially available cultured adult human dermal fibroblasts (HDFa) vs. multi-drug resistant bacteria
Project description:Gene expression analysis of BJhTERT RhoA-KO and PC3 mRFP was performed before and after six days confrontation in co-cultivation. Analyses of original and control fibroblasts was also performed. To identify molecules that may mediate the tumor promoting effect of RhoA knock out fibroblasts, we performed gene expression analysis of BJhTERT RhoA-KO and PC3 mRFP cells before the confrontation and then after six days after it, using Affymetrix Whole Transcript Assay platform.
Project description:To investigate the cooperative function of dermal fibroblast and mast cell in inducing mast cell tolerance to commensals We then performed gene expression profiling analysis using data obtained from SC RNA seq of mast cells, dermal fibroblast and and mast cells in co culture with dermal fibroblasts .
Project description:Gene expression analysis of BJhTERT RhoA-KO and PC3 mRFP was performed before and after six days confrontation in co-cultivation. Analyses of original and control fibroblasts was also performed. To identify molecules that may mediate the tumor promoting effect of RhoA knock out fibroblasts, we performed gene expression analysis of BJhTERT RhoA-KO and PC3 mRFP cells before the confrontation and then after six days after it, using Affymetrix Whole Transcript Assay platform. Four days old fibroblast monolayer was confronted with PC3 mRFP tumor cells, plated in ratio 1:30 to number of plated fibroblasts. After six days of confrontation cells were sorted by FACS (Fluorescence-activated cell sorting). Total RNA was isolated from each confronted and non-confronted cells with kit. Then 150 ng of total RNA were used for transcriptomic analysis.for RNA extraction and hybridization on Affymetrix microarrays
