Project description:This project aims to identify novel RNA binding proteins in the baker's yeast, Saccharomyces cerevisiae. Since interactions between RNAs and proteins may be transient, yeast cells were crosslinked with UV light at 254 nm which promotes the covalent link between proteins and RNAs. After this, polyadenylated mRNAs were purified via oligo(dT) coupled to magentic beads under stringet conditions. Finally, samples were subjected to mass spectrometry analysis. To rule out the possibility of RNA-independent binding we also analysed other samples: i) samples digested with RNase one; ii) samples where we performed competition assays with polyadenylic acid.

2015-10-26 | PXD002293 | Pride

Expression data from Saccharomyces cerevisiae Met30 temperature-sensitive strain

Project description:The target of rapamycin (TOR) plays a central role in eukaryotic cell growth control. With prevalent hyper-activation of the mTOR pathway in human cancers, novel strategies to enhance TOR pathway inhibition are highly desirable. We used a yeast-based high-throughput chemical genetic screen to identify small-molecule enhancers of rapamycin (SMERs) and used whole genome expression analysis to identify their mechanisms of action.

2011-06-30 | GSE22270 | GEO

Expression data from Saccharomyces cerevisiae Met30 temperature-sensitive strain

2011-06-30 | GSE22270 | GEO

Saccharomyces cerevisiae strain:BY4742

Project description:Saccharomyces cerevisiae strain:BY4742 Raw sequence reads

| PRJNA302907 | ENA

Saccharomyces cerevisiae strain:BY4742

Project description:The dynamic response to an activator is dictated by nucleosome and TBP stability differences between TFIID house-keeping and SAGA regulated promoters

| PRJNA321111 | ENA

Saccharomyces cerevisiae strain:BY4742

Project description:single-cell Saccharomyces cerevisiae raw sequence reads

| PRJNA305338 | ENA