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Expression data from prostate cancer and osteoblast co-cultures

ABSTRACT: Expression data from prostate cancer and osteoblast co-cultures

OTHER RELATED OMICS DATASETS IN: GSE73044

PROVIDER: PRJNA296005 | ENA |

REPOSITORIES: ENA

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Expression data from prostate cancer and osteoblast co-cultures

Project description:Dynamic interaction between prostate cancer and the bone microenvironment is a major contributor to metastasis of prostate cancer to bone. In this study we utilized an in-vitro co-culture model of PC3 prostate cancer cells and osteoblasts followed by microarray based gene expression profiling to identify previously unrecognized prostate cancer-bone microenvironment interactions. Factors secreted by PC3 cells resulted in the up-regulation of many genes in osteoblasts associated with bone metabolism and cancer metastasis, including Mmp13, Il-6 and Tgfb2, and down-regulation of Wnt inhibitor Sost. To determine whether altered Sost expression in the bone microenvironment has an effect on prostate cancer metastasis, we co-cultured PC3 cells with Sost knockout (SostKO) osteoblasts and wildtype (WT) osteoblasts and identified several genes differentially regulated between PC3-SostKO osteoblast co-cultures and PC3-WT osteoblast co-cultures. Co-culturing PC3 cells with WT osteoblasts up-regulated cancer-associated long noncoding RNA (lncRNA) MALAT1 in PC3 cells. MALAT1 expression was further enhanced when PC3 cells were co-cultured with SostKO osteoblasts and treatment with recombinant Sost down-regulated MALAT1 expression in these cells. Our results suggest that reduced Sost expression in the tumor microenvironment may promote bone metastasis by up-regulating MALAT1 in prostate cancer.

2015-09-30 | GSE73044 | GEO

Expression data from human osteoblast cultures

Project description:Expression data from human osteoblast cultures

| PRJNA660940 | ENA

Expression profiling of stroma cells from prostate cancer co-cultures, with or without TGF-beta signaling

Project description:Prostate stroma-specific TGF-beta signaling induces morphological changes in LNCaP cells. We have previously shown that stromal TGF-beta signaling regulates prostate tumor growth. To further delineate the underlying mechanisms, we generated LNCaP cells overexpressing an HA-tagged constitutively activate TGF-beta1 ligand (LNCaP-HA-TGF-β1(a)) and control LNCaP cells (LNCaP-Ctrl), and performed in vitro co-cultures of LNCaP-HA-TGF-β1(a) and LNCaP-Ctrl cells on top of the confluent HPS-19I cells, a human prostate stromal cell line. Since LNCaP cells are defective in TGF-beta receptor I (TbetaRI / ALK-5) that is essential for mediating TGF-beta signaling, only HPS19I cells are able to respond to TGF-beta ligand in these co-cultures. This provides a unique opportunity to study how prostate stromal cell-specific TGF-beta signaling regulates PCa biology. To identify the prostate epithelia-specific gene that was regulated by prostate stromal TGF-beta signaling, we also treated HPS19I cells using conditioned media collected from LNCaP- HA-TGF-β1(a) cells and LNCaP-Ctrl cells cultured in RPMI1640 supplemented with 0.2% FBS. After 6 days of treatment, we extracted total RNA from these HPS19I cells and performed microarray.

2014-11-12 | GSE51622 | GEO

Expression profiling of co-cultures of prostate cancer cells and stroma cells, with or without TGF-beta signaling

2014-11-12 | GSE51617 | GEO

Expression data from human osteoblast cultures

Project description:Studies in Aotearoa New Zealand found higher bone mineral density and lower rate of hip fracture in people of Polynesian ancestry compared to Europeans. We hypothesised that differences in osteoblast gene expression contribute to the differences in bone properties between the two groups, and aimed to identify mechanisms that underlie the bone phenotype.

2020-09-03 | GSE157322 | GEO

SFRP2 Overexpression Induces an Osteoblast-Like Phenotype in Prostate Cancer Cells

Project description:Abstract: Prostate cancer bone metastasis is still one of the most fatal cancer diagnosis for men. Survival of the circulating prostate tumor cells and their adaptation strategy to survive in the bone niche is the key point to determining metastasis in early cancer stages. The promoter of SFRP2, a WNT signaling regulator, is hypermethylated in many cancer types including prostate cancer. Moreover, SFRP2 can positively modulate osteogenic differentiation in vitro and in vivo. Here, we showed SFRP2 overexpression in the prostate cancer cell line PC3 induces an epithelial mesenchymal transition (EMT), increases the attachment, and modifies the transcriptome towards an osteoblast-like phenotype (osteomimicry) in a collagen 1‑dependent manner. Our data reflects a novel molecular mechanism how metastasizing prostate cancer cells might increase their chance to survive within bone tissue.

2022-12-16 | GSE217979 | GEO

Co-cultures of prostate cancer cells and stroma cells, with or without TGF-beta signaling

Project description:Co-cultures of prostate cancer cells and stroma cells, with or without TGF-beta signaling

| PRJNA224103 | ENA

Comparison of extracellular matrix in prostate tissue samples and in cultures of primary fibroblasts from prostate cancer patients

Project description:xtracellular matrix from prostate tissue samples and from cultures of primary fibroblasts of the same samples from individuals was analysed by mass spectrometry.

2018-03-13 | PXD006562 | Pride

RNA-sequencing of prostate cancer xenografts, their 3D cultures and prostate cancer cell-lines.

Project description:RNA-sequencing of prostate cancer xenografts, their 3D cultures and prostate cancer cell-lines.

2021-09-29 | E-MTAB-9930 | biostudies-arrayexpress

Expression data from Low Temperature Plasma-treated primary prostate cultures

Project description:Prostate basal epithelial cultures generated from patient normal and cancer tissue were treated with LTP for 3 minutes and RNA harvested at 2hrs post-LTP. Microarrays were used to analyze whole transcriptome changes between untreated and LTP-treated prostate cells.

2018-08-27 | GSE119052 | GEO

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