Project description:Mouse peritoneal macrophages were transfected with 80-120 nM miRIDIAN miRNA mimics (miR-mimic-33/miR-mimic-33*) or with 80-120 nM miRIDIAN miRNA inhibitors (anti-miR-33 ASO/anti-miR-33*ASO) Control samples were treated with an equal concentration of a non-targeting control mimics sequence (control mimic) or inhibitor negative control sequence (control aso), to control for non-specific effects in miRNA experiments.
Project description:A microarray experiment was performed to investigate changes in gene expression induced in the Huh7.5 hepatoma cell line following treatment for 24 hours with recombinant human Bone Morphogenetic Protein 6 (BMP6) (18 nM).
Project description:Transcriptional profiling of human hepatoma cell lines comparing control uninfected Huh7.5 cells with Huh7.5 cells persistently infected with HCV (HPI cell). The latter maintains and produces HCV.
Project description:This experiment is designed to investigate the impact of exosomal miR-145-5p on the functional pathway and molecules on RPTEC cells. Data-independent acquisition (DIA) proteomics was conducted in the RPTEC cells transfected with miR-145-5p mimic or scramble control.
Project description:To experimentally investigate the function of hsa-miR-511-5p in AML monoblasts, we employed miRNA mimic mediated up-regulation of miR-511 in THP1 cells and subsequently analyzed a comprehensive gene expression profile. 4 samples of two independent miRNA mimic experiments were analyzed. THP1 monoblasts were transfected with 30 nM of Ambion Pre-miR miRNA Precursors (hsa-miR-511-5p AM10237 or negative Control #1 AM17110, Life Technologies).
Project description:Hepatocellular carcinoma (HCC) is a cancer with global impact and largely refractory to current treatments. Novel treatment options are therefore urgently needed. MicroRNAs play important regulatory roles in HCCs and are emerging as promising therapeutic options against HCC. We identified tumor suppressor miRNAs that may attenuate tumor development and contribute to HCC regression. We identified miR-342-3p as a promising tumor suppressor miRNA. To understand how miR-342-3p affects the global landscape of gene expression, we transfected Huh7 human hepatoma cells with either the scramble control, or a mimic for miR-342-3p and performed mRNA expression profiling.
Project description:Transcriptional profiling of human monocyte-derived dendritic cells (MDDCs), comparing cells transfected with miR-29a mimic, or miR-29b mimic, or negative control.
Project description:The miR-99 family is one of the evolutionarily most ancient microRNA families, and it plays a critical role in development. There are 3 members of the miR-99 family in humans (miR-99a, miR-99b, miR-100). Recent studies suggested that miR-99 family also regulates various physiological processes in adult tissues, such as wound healing, and a number of diseases processes, including cancer. Here, we aim to identify gene expression changes mediated by miR-99 family in epithelial cells. HaCaT cells and 1386Ln cells were transfected with miR-100 mimic or with negative control mimic (Dharmacon), the total RNA was isolated using miRNeasy Mini kit (Qiagen), and labeled and hydridized to the Affymetrix GeneChip Human Gene 1.0 ST arrays according standard protocol.
