Project description:It has been performed a genome-wide analysis of gene expression of the root-colonizing bacterium Pseudomonas putida KT2440 in the rhizosphere of corn (Zea mays var. Girona. To identify reliable rhizosphere differentially expressed genes, rhizosphere populations of P. putida bacteria cells were compared with three alternative controls: i) planktonic cells growing exponentially in rich medium (LB), ii) planktonic cells in stationary phase in LB, and iii) sessile populations established in sand microcosms, under the same conditions used to grow inoculated corn plants.
Project description:Effect of the inactivation of locus PP4959 upon gene expression of P. putida KT2440 in the rhizosphere of corn (Zea mays var. Girona). This locus encodes the unique dual GGDEF/EAL domains response regulator in KT2440. To identify those genes with altered expression, cells were recovered from the rhizosphere six days after inoculation of gnotobiotic seedlings.
