Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA300525 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The effects of Gata4 inhibition on global gene expression in TM4 Sertoli cells was analyzed. Using microarray analysis we identified a set of genes that are downregulated or upregulated following siRNA-mediated silencing of Gata4 in the murine Sertoli cell line TM4. Among the downregulated genes were regulators of blood-testtis barrier function and lactate metabolism. TM4 cells were transfected with non-targeting (NT) siRNA and Gata4 siRNA respectively. 72h post transfection cells were lysed and RNA was extracted.
2016-03-17 | GSE74471 | GEO
Project description:The effects of Gata4 inhibition on global gene expression in TM4 Sertoli cells was analyzed. Using microarray analysis we identified a set of genes that are downregulated or upregulated following siRNA-mediated silencing of Gata4 in the murine Leydig tumor cell line mLTC-1. Among the downregulated genes were enzymes of the androgen biosynthetic pathway. TM4 cells were transfected with non-targeting (NT) siRNA and Gata4 siRNA respectively. 72h post transfection cells were lysed and RNA was extracted. n=3 of each sample group; control samples are samples 3-6 (TM4 cells treated with non-targeting siRNA)
2016-03-17 | E-GEOD-74471 | biostudies-arrayexpress
Project description:Genome-wide analysis of gene expression in mLTC-1 cells following siRNA-mediated Gata4 inhibition
| PRJNA267448 | ENA
Project description:The effects of Gata4 inhibition on global gene expression in mLTC-1 cells was analysed. Using microarray analysis we identified a set of genes that are downregulated or upregulated following siRNA-mediated silencing of Gata4 in the murine Leydig tumor cell line mLTC-1. Among the downregulated genes were enzymes of the androgen biosynthetic pathway. mLTC-1 cells were transfected with non-targeting (NT) siRNA and Gata4 siRNA respectively. 72h post transfection cells were lysed and RNA was extracted.
2015-02-23 | GSE63320 | GEO
Project description:The effects of Gata4 inhibition on global gene expression in mLTC-1 cells was analysed. Using microarray analysis we identified a set of genes that are downregulated or upregulated following siRNA-mediated silencing of Gata4 in the murine Leydig tumor cell line mLTC-1. Among the downregulated genes were enzymes of the androgen biosynthetic pathway. mLTC-1 cells were transfected with non-targeting (NT) siRNA and Gata4 siRNA respectively. 72h post transfection cells were lysed and RNA was extracted. n=3 of each sample group; control samples are samples 3-6 (mLTC-1 cells treated with non-targeting siRNA)
2015-02-23 | E-GEOD-63320 | biostudies-arrayexpress
Project description:This study was designed to investigate the toxicological effects of MEHP on TM3 Leydig and TM4 Sertoli cells.
2024-05-25 | GSE243503 | GEO
Project description:ERM knockout mice were produced. Testis develop a Sertoli cell only syndrome, which appears complete by 10 weeks of age in male ERM knockout mice. Three sets of Affymetrix comparisons were made. #1 and #2, Wt or ERM knockout testis from 4 week old males, totaly RNA. This time preceeds any significant histologic difference between the wt or knockout. The results was the finding that only a very specific set or markers for spermatogonial STEM CELLs were found to be greatly reduced in the ERM knockout. #3 and #4. The sertoli cell line TM4 was transduced with a retroviral ERM. The wild type TM4 cell line was compared to this TM$-ERM cell line, with the finding that only a few genes were increased in the TM4-ERM line. #5, #6 and #7. Primary Sertoli cells were purified from 4 week old wild type or ERM knockout or from 10 week old ERM knockout mice and compared. Keywords: other
2005-04-01 | GSE2205 | GEO
Project description:Zearalenone (ZEA) is a non-steroidal xenoestrogen mycotoxin produced by many Fusarium fungal species, which are common contaminants of cereal crops destined for worldwide human and animal consumption. ZEA has been linked to various male reproduction problems including decreased fertility potential. In this study, the direct impact of ZEA on the immature Sertoli TM4 cell line was evaluated. Results showed that high concentration of ZEA increase reactive oxygen species via the activation of MAPK signaling. Transcriptome analysis was performed on TM4 cell line treated with ZEA and genes involved in sex differentiation (Fgfr2, Igf1, Notch1, Sox9) and extracellular matrix (ECM) formation (Ctgf, Fam20a, Fbn1, Mmp9, Postn, Sparcl1, Spp1) were identified at the center of the functional protein association network suggesting that ZEA could be detrimental to the early steps of Sertoli cell differentiation.
2022-03-07 | GSE185111 | GEO
Project description:ERM knockout mice were produced. Testis develop a Sertoli cell only; syndrome, which appears complete by 10 weeks of age in male ERM knockout mice. Three sets of Affymetrix comparisons were made. #1 and #2, Wt or ERM knockout testis from 4 week old males, totaly RNA. This time preceeds any significant histologic difference between the wt or; knockout. The results was the finding that only a very specific set or; markers for spermatogonial STEM CELLs were found to be greatly reduced in the ERM knockout. #3 and #4. The sertoli cell line TM4 was transduced with a retroviral; ERM. The wild type TM4 cell line was compared to this TM$-ERM cell line,; with the finding that only a few genes were increased in the TM4-ERM line. #5, #6 and #7. Primary Sertoli cells were purified from 4 week old wild; type or ERM knockout or from 10 week old ERM knockout mice and compared.
2008-06-11 | E-GEOD-2205 | biostudies-arrayexpress
Project description:Here, we show that adult cardiac fibroblasts express the cardiomyocyte transcription factors GATA-4 and GATA-6 to promote adaptive remodeling in pressure overload induced cardiac hypertrophy. Using a mouse model with specific single or double deletion of Gata4 and Gata6 in stress activated fibroblasts, we found a reduced myocardial capillarization in mice with Gata4/6 double deletion following pressure overload, while single deletion of Gata4 or Gata6 had no effect. Importantly, we confirmed the reduced angiogenic response using an in vitro co-culture system with Gata4/6 deleted cardiac fibroblast and endothelial cells. A comprehensive RNA-sequencing analysis revealed an upregulation of anti-angiogenic genes upon Gata4/6 deletion in fibroblasts, and siRNA mediated downregulation of these genes restored endothelial cell growth. In conclusion, we identified a novel role for the cardiac transcription factors GATA4 and GATA6 in heart fibroblasts, where both proteins act in concert to promote myocardial capillarization and heart function by directing intercellular crosstalk.
2021-04-28 | GSE155358 | GEO