Project description:Basil downy mildew (BDM) caused by Peronospora Belbahrii leads to losses in sweet basil cultivation across the world. Though resistant cultivars of basil exist, the formation of sterile offspring and the introduction of unwanted phenotypic and chemotypic traits slows breeding. Previous work by the Simon lab at Rutgers University identified pair of sweet basil cultivars; one resistant to BDM, MRI, and one susceptible, SB22. They predicted that three genes in MRI confer increased BDM resistance. RNA from infected MRI and SB22 plants was harvested during the first 3 days of infection at 4 timepoints in order to capture as many early phases of plant-pathogen interaction as possible. The goal is to develop resistance markers for use in breeding experiments.
Project description:This experiment has been annotated by TAIR (http://arabidopsis.org). We examined transcript profiles triggered by three different arabidopsis R genes that recognize distinct Peronospora parasitica isolates. Experimenter name = Thomas Eulgem; Experimenter phone = 43 1 4277 54622; Experimenter fax = 43 1 4277 9546; Experimenter department = Institute of Microbiology and Genetics; Experimenter address = Institute of Microbiology and Genetics; Experimenter address = Dr. Bohrgasse 9; Experimenter address = Vienna; Experimenter zip/postal_code = A-1030; Experimenter country = Austria Experiment Overall Design: 3 samples were used in this experiment
