Project description:Expression microarray of livers from adult zebrafish: control, overfed, and ahcy+/- Comparison of expression from livers obtained from ahcy+/+, overfed ahcy+/+, ahcy+/- control and ahcy+/- overfed.
Project description:Expression microarray of livers from adult zebrafish: control, overfed, and ahcy+/- Comparison of expression from livers obtained from ahcy+/+, overfed ahcy+/+, ahcy+/- control and ahcy+/- overfed. Adult zebrafish, ahcy+/+ and +/-, were given a regular diet or were overfed for a month. Livers were removed, RNA isolated, and expression microarray was performed.
Project description:Lion-head goose is the only large goose species in China, and it was one of the largest goose species in the world. Our previous study firstly reported a chromosome-level genome assembly of Lion-head goose (Anser cygnoides), a native breed in South China, through the combination of PacBio, Bionano, and Hi-C technologies. The fat content of foie gras is augmented during its preparation due to the special feeding regimen. Lion-head geese have a strong tolerance of massive energy intake and show a priority of fat accumulation in liver tissue. In this study, we studied for the first time the important differential genes that regulate fatty liver in Lion-head goose. After high-intake feeding, the fatty livers of Lion-head geese were distinctly characterized. The revelation of gene regulation is an important basis for the study of liver development and molecular characteristics for the Lion-head goose. To analyze the excellent fatty liver performance of Lion-head goose at the molecular level, we performed whole transcriptome analysis by high-throughput RNA sequencing to analyze the key regulatory genes that determine the fatty livers in high-intake feeding group compared with the normal livers in normally-fed Lion-head geese. We identified 716 differentially expressed mRNAs, 145 differentially expressed circRNAs, and 39 differentially expressed lncRNAs in the fatty livers in high-intake feeding group compared with the normal livers in normally-fed Lion-head geese, including upregulated and downregulated genes, respectively. GO enrichment analysis showed that these genes were significantly enriched in molecular function, involved in extracellular regions, DNA-binding transcription factor activity, extracellular matrix, heme binding and other life activities. We chose differentially expressed genes involved in either upregulation or downregulation, and we additionally confirmed the accuracy of sequencing at the RNA level. In summary, our research suggested that these differentially expressed genes may play important roles in fatty liver development in Lion-head goose. However, the functions and mechanisms of these significantly differentially expressed genes should be investigated in future studies.
2023-11-16 | GSE243829 | GEO
Project description:IIB/RAD sequencing of overfed Landes Geese
Project description:We report the transcriptomes (polyadenylated mRNAs over 200bp) of animals overfed through intragastric tubes either in the overfed state (OIO) or 24 hours after cessation of overfeeding (OIOneg). We also report transcriptomes of weight-matched mice either fed (ALO, or fasted 24 hours (ALOneg).