Project description:Total RNA versus genomic DNA hybridization on custom arrays designed for all Candida albicans genes Overall design: Total RNA was collected in mid-log phase from Candida albicans cells grown in rich medium (abbreviated CM, in house recipe). RNA was then converted to cDNA, Cy3-labeled and hybridized competitively against Cy5 labeled genomic DNA from Candida albicans
Project description:Map ORC binding sites to identify replication origins in C. albicans by using polyclonal ORC antibodies (gift from Stephen Bell Lab). Due to the unsynchronized nature of Candida cells, log-phase cultures were taken to perfoem ChIP-chip experiments to find the genome-wide ORC binding sites. ChIP-chip experiments from log-phase C. albicans culture. 7 different unsynchronized log-phase cultures as replicates.