Project description:Elucidation of the involvement of MOR (morphogenesis-related NDR kinase network) pathway in the gene expression during appressorium development in planta, transcriptome analyses at the stage of appressorium formation (4 hours post inoculation) in vitro and in planta were performed and revealed that MOR is responsible for the expression of a subset of the plant signal induced genes with potential roles in pathogenicity.
Project description:Species from the genus Colletotrichum are the causal agents of anthracnose which contribute to significant losses to the production of commercially grown crops. The genomes of Colletotrichum orbiculare, which infects cucurbits and Nicotiana benthamiana, as well as Colletotrichum gloeosporioides, which infects a wide range of fruits and vegetables, were sequenced. A custom microarray was designed for Colletotrichum orbiculare and used to assess gene expression during infection of Nicotiana benthamiana. Gene expression of Colletotrichum orbiculare growing on its host Nicotiana benthamiana was assessed at 24 hours post inoculation, 3 days post inoculation and 7 days post inoculation. Mycelia growing in vitro and ungerminated conidia were used as controls. Three replicates were performed for each time point.
Project description:To elucidate the involvement of MOR (morphogenesis-related NDR kinase network) pathway in regulation of genes that are specifically expressed during appressorium development in planta, transcriptome analyses at the stage of appressorium formation (4 hours post inoculation) and in planta were performed and revealed that MOR is responsible for the expression of a subset of the plant signal induced genes with potential roles in pathogenicity.
Project description:Time course of C. orbiculare infection of the host plant, N. benthamiana, and comparison to in vitro grown tissue (conidia and in vitro grown hyphae) to identify genes that contribute to the infection process by transcript expression profile.
Project description:Colletotrichum orbiculare Whi2, yeast stress response Whi2 homolog, is involved in switch from biotrophic to necrotrophic stage. To elucidate downstream genes regulated by Co Whi2, we have conducted DNA microarray. About 3100 genes were up or down regulated in the Co whi2Δ mutant compared with the wild-type. In particularly, 44 genes among up-regulated 58 genes in the Co whi2Δ mutant are ribosomal protein related gene. Eukaryote is widely conserved TOR (Target Of Rapamycin) which is known to regulator of ribosomal gene expression. To elucidate whether up-regulated ribosomal genes in the Co whi2Δ mutant are regulated by TOR activity, we have conducted DNA microarray in the Co whi2Δ mutant treated with rapamycin inhibiting TOR activity. The enormous ribosomal gene expression in the Co whi2Δ mutant treated with the rapamycin is lower than that without rapamycin treatment.
Project description:Colletotrichum orbiculare Whi2, yeast stress response Whi2 homolog, is involved in switch from biotrophic to necrotrophic stage. To elucidate downstream genes regulated by Co Whi2, we have conducted DNA microarray. About 3100 genes were up or down regulated in the Co whi2Î mutant compared with the wild-type. In particularly, 44 genes among up-regulated 58 genes in the Co whi2Î mutant are ribosomal protein related gene. Eukaryote is widely conserved TOR (Target Of Rapamycin) which is known to regulator of ribosomal gene expression. To elucidate whether up-regulated ribosomal genes in the Co whi2Î mutant are regulated by TOR activity, we have conducted DNA microarray in the Co whi2Î mutant treated with rapamycin inhibiting TOR activity. The enormous ribosomal gene expression in the Co whi2Î mutant treated with the rapamycin is lower than that without rapamycin treatment. In gene expression of the Co whi2Î mutant, the wild-type and the Co whi2Î mutant infecting on cucumber cotyledons were assessed at 4 hours post-inoculation. In gene expression of the Co whi2Î mutant with rapamycin treatment, Co whi2Î mutant treated with 100nM rapamycin and Co whi2Î mutant without rapamycin treatment infecting on cucumber cotyledons were assesed at 4 hours post-inoculation. Four replication were performed for each experiments.
Project description:Transcriptome of 4 developmental stages of Colletotrichum higginsianum during infection of Arabidopsis thaliana 3 biological replicates per stage. The four stages are : in vitro apressoria (VA), penetrating apressoria (AP), Biotrophic hyphea from the biotrophic phase (BH) and the switch from biotrophic hyphea to necrotrophic phase(SW). Each biological replicate of the last three stages, the in planta stages, was two fold deeper due to plant contamination (Arabidopsis thaliana)
