Project description:Mrakia psychrophlila is a psychrophilic fungus widely spread all over the world. The cold adaptation mechanism of Mrakia psychrophila is unknown. Using RNA sequencing, differentially expressed genes of Mrakia psychrophila cultured at 4, 12 and 20 degree centigrade are identified. The result may be benefit for understanding cold adaptation of microorganisms.
Project description:Comprehensive and quantitative information of the psychrophile proteome is an important source for understanding of the cold adaptation mechanism under low growth temperature. Mrakia psychrophila, a obligate fungus, was selected to quantitatively evaluate its protein abundance changes in response to three different temperatures. With optimized procedures of isobaric tags for relative and absolute quantitation quantitative proteomics(iTRAQ), high quality of the quantitative information of the peptides identified were acquired. In total, 1673 unique proteins were identified and defined 129 proteins as the temperature-dependent proteins. Function analysis revealed that energy metabolism is important for temperature adaptaion.
