Project description:To explore the methylation altering effects of oil on developing fish, we exposed larval Cyprinodon variegatus to oil for 48 h and used immunoprecipitation coupled with high-throughput sequencing (MeDIP seq) to evaluate genome-wide methylation changes.
Project description:Fifteen adult male Cyprinodon variegatus were exposed individually to 10µg/L of bisphenol A (BPA) for 7 days in 1.8L vessels and other fifteen to 10 µg/L of BPA metabolite, 4-methyl-2,4-bis(4-hydroxyphenyl)pent-1-ene (MBP) under the same conditions. Dimethyl sulfoxide (DMSO) was used as a carrier at 1µL/100mL and was added at the same concentration to the solvent control (n = 15). 90% daily water renewal was done after monitoring the culture conditions, that were: temperature 18.8 ± 0.2°C, pH 7.94±0.03, dissolved oxygen saturation 99.3% ± 1.1 and 16:8 hours of light:dark photoperiod. BPA, MBP and DMSO concentrations were adjusted daily after water renewal.
