Project description:Transcriptome analysis reveals the response mechanism of Frl-mediated resistance to Fusarium oxysporum f. sp. radicis-lycopersici (FORL) infection in tomato
Project description:In plant pathogenic fungi, conditionally dispensable (CD) chromosomes are often associated with virulence, but not viability. Such virulence-associated CD chromosomes carry genes encoding effectors and/or host-specific toxin biosynthesis enzymes, potentially important for determining host specificity. Fusarium oxysporum causes devastating diseases of more than 100 plant species. In particular, F. oxysporum f. sp. conglutinans (Focn) can infect Brassicaceae plants including Arabidopsis and cabbage. Here we show that Focn has multiple CD chromosomes involving in not only virulence but also vegetative growth, which is an atypical feature of known CD chromosomes. Among them, we identified specific CD chromosomes that are required for virulence to either Arabidopsis, cabbage, or both. We revealed that a pair of effectors encoded in one of the CD chromosomes is required for suppression of the Arabidopsis-specific phytoalexin-based immunity. The effector pair is highly conserved in F. oxysporum isolates capable of infecting Arabidopsis. This study provides insights into how host specificity of F. oxysporum is determined by a pair of effector genes on a CD chromosome.
Project description:Tandem Mass Tag (TMT)-based quantitative proteomic analysis of tomato soil borne pathogen Fusarium oxysporum f. sp. radicis-lycopersici growth, and metabolism when treated with plant natural volatile organic compounds linalool. The Forl strain was cultured on PDA supplied with 0.8 mL/L linalool for 6 days at 25°C. The fungal strain on PDA supplied with only 0.1% Tween80 was cultured as the control. Three biological replicates were established for each treatment.