Project description:Lysine acetylation is a major post-translational modification that plays an important regulatory role in almost every aspects in both eukaryotes and prokaryotes. Bacillus amyloliquefaciens, a Gram-positive bacterium, is very effective for the control of plant pathogens. Here, we conducted the first lysine acetylome in B. amyloliquefaciens through a combination of highly sensitive immune-affinity purification and high-resolution LC−MS/MS. Overall, we identified 3268 lysine acetylation sites in 1254 proteins. Acetylated proteins are associated with a variety of biological processes and a large fraction of these proteins are involved in metabolism. These data serves as an important resource for further elucidation of the physiological role of lysine acetylation in B. amyloliquefaciens.
Project description:Root exudates play an important role in plant-microbe interaction. The transcriptional profilings of plant growth-promoting rhizobacteria Bacillus amyloliquefaciens SQR9 in response to maize root exudates under static condition, were investigated by an Illumina RNA-seq for understanding the regulatory roles of the root exudates.
Project description:The effect of maize seed (Zea mays L. var. Surprise) exudates on Bacillus amyloliquefaciens FZB42 cultures grown to OD600 = 1.0 or 3.0 was tested against a negative seed exudate control sample.
Project description:The transcriptomic response of Bacillus amyloliquefaciens FZB42 to maize root exudates at OD600=3.0. This is a comprehensive analysis using the data of six microarray experiments (Exp1-2-3 and ExpABC respectively, 18 hybridization in total).
