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Photorhabdus laumondii subsp. laumondii TTO1

ABSTRACT: identification by RNA-Seq of PhoP and polymyxin B resistant regulons in Photorhabdus luminescens TT01.

PROVIDER: PRJNA307974 | ENA |

REPOSITORIES: ENA

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Photorhabdus laumondii subsp. laumondii TTO1

Project description:DNA adenine methyltransferase (Dam) overexpression impairs Photorhabdus luminescens motility and virulence

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identification by RNA-Seq of PhoP and polymyxin B resistant regulons in Photorhabdus luminescens TT01.

Project description:A phoP mutant of the entomopathogenic bacterium P. luminescens is attenuated in virulence in insects and susceptible to antimicrobial peptides such as Polymyxin B. The first goal of this study is to compare transcriptomes of the phoP mutant and wild type strain to identify the PhoP regulon. (i) We first compared both strains grown in LB medium. (ii) As we know that low Mg conditions induce expression of PhoP-dependent genes, we also compared transcriptomes of the phoP and wild-type strain after growth in M9 low Mg and in M9 High Mg. To decipher the polymyxin B regulon, comparisons of transcriptomes of the wild type strain grown in LB medium with or without addition of polymyxin B were also performed

2017-03-10 | GSE76559 | GEO

Photorhabdus laumondii subsp. laumondii TTO1

Project description:Transcriptionnal analysis of a Photorhabdus luminescens TT01 variant, named VAR*, which is a colonial and phenotypic variant displaying delayed pathogenicity in the insect, Spodoptera littoralis.

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Gene expression of a Photorhabdus M-form variant relative to the wild type P-form cells

Project description:Photorhabdus luminescens bacteria alternate lifestyles between its pathogenic insect host and its mutualistic nematode host. We found that Photorhabdus changes radically from the insect pathogenic form (P-form) to slow growing small cells when initiating mutualism (M-form). Here we characterize the gene expression of the M-form relative to the P-form.

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Cappable seq of Photorhabdus luminescens TTO1

Project description:Cappable seq of Photorhabdus luminescens TTO1

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Photorhabdus laumondii subsp. laumondii TTO1

Project description:Gene expression of a Photorhabdus M-form variant relative to the wild type P-form cells

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DNA adenine methyltransferase (Dam) overexpression impairs Photorhabdus luminescens motility and virulence

Project description:Dam, the most described bacterial DNA-methyltransferase, is widespread in gamma-proteobacteria. Dam DNA methylation can play a role in various genes expression and is involved in pathogenicity of several bacterial species. In the entomopathogenic bacterium Photorhabdus luminescens, a dam ortholog was identified. Overexpression of dam in P. luminescens did not impair growth ability in vitro. In contrast, compared to a control strain harboring an empty plasmid, a significant decrease in motility was observed in the dam-overexpressing strain. In addition, the dam-overexpressing P. luminescens strain showed a delayed virulence compared to that of the control strain after injection in larvae of the lepidopteran Spodoptera littoralis. These results reveal that Dam plays a major role during P. luminescens insect infection.

2017-10-04 | GSE100650 | GEO

Photorhabdus laumondii subsp. laumondii strain:HP88

Project description:Photorhabdus luminescens subsp. laumondii HP88 genome sequencing and assembly

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Transcriptionnal analysis of a Photorhabdus luminescens TT01 variant, named VAR*, which is a colonial and phenotypic variant displaying delayed pathogenicity in the insect, Spodoptera littoralis.

Project description:Photorhabdus luminescens lives in a mutualistic association with entomopathogenic nematodes and is pathogenic for insects. Variants of Photorhabdus frequently arise. VAR* is a colonial and phenotypic variant displaying delayed pathogenicity in the insect, Spodoptera littoralis. In this study, we evaluated the role of transcriptomic modulation in determining the phenotypic variation and delayed pathogenicity of VAR* with respect to the corresponding wild-type form, TT01alpha. A P. luminescens microarray was used to determine changes in transcript profiles in the exponential and stationary phase growth phase of VAR* and TT01alpha grown in LB broth.

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Photorhabdus laumondii subsp. laumondii strain:DJC

Project description:Photorhabdus luminescens subsp. laumondii strain DJC Genome sequencing and assembly

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