Project description:Penicillium digitatum is the pathogen of Green mold in Postharvest citrus. After inoculating Penicillium digitatum into the wound of citrus to infect it, transcriptome sequencing was carried out and compared with the results of transcriptome sequencing of Penicillium digitatum before inoculation in order to screen the differentially expressed genes and reveal its infection mechanism.
Project description:In order to obtain genetically engineering strain of Penicillium oxalicum with high Raw Starch Digesting Glucoamylase production,we deleted PoxCxrC and overexpressed POX_f08097 in Penicillium oxalicum TE4-10 ,and we want to investigating the function of deletion of PoxCxrC and overexpression of POX_f08097 at transcriptional level.
Project description:A transcriptomic approach was implemented using two Penicillium species to identify genes related to fungal aggressiveness in apple fruit and loci contained in ungerminated conidia. Total RNA was isolated from ungerminated conidia and decayed apple fruit infected with P. expansum R19 (aggressive) or P. polonicum RS1 (weak). There were 2,442 differentially expressed genes (DEGs) between the R19 and RS1 in apple and comparisons within species between apple and conidia revealed 4,404 DEGs for R19, and 2935 for RS1, respectively. Gene ontology (GO) revealed differential regulation in fungal transport and metabolism genes expressed during decay, suggesting a flux in nutrient acquisition and detoxification strategies. In R19, the oxidoreductase GO category comprised 20% of all groups differentially expressed in decayed apple verses ungerminated conidia in addition to those involved in hydrogen peroxide metabolism. Ungerminated conidia from both species showed higher expression of genes encoding the glyoxylate shunt and beta-oxidation, specifying the earliest metabolic requirements for germination
