Project description:In this study, we interrogated the role of DNA methylation in HSPC generation by taking advantage of dnmt1 knockout/knockdown embryos in zebrafish. First, we generated a comprehensive DNA methylation landscape of EHT, which revealed gradually hypermethylated regions associated with vasculogenesis. Taking advantage of dnmt1-deficient embryos, we showed that the decreased DNA methylation blocked HSPC emergence. Mechanistically, we demonstrated that the decreased DNA methylation increased the expression of arterial genes and Notch signaling, thus contributing to defects in the EHT in dnmt1-deficient embryos. Herein, we identified that DNA methylation, as epigenetic regulator, participates in the negative modulation of Notch signaling through inhibiting transcription during HSPC generation in zebrafish.

2022-05-10 | GSE189072 | GEO

Danio rerio

Project description:microRNA expression during early zebrafish development

| PRJNA92137 | ENA

Danio rerio

Project description:Ribosome profiling of early zebrafish embryos -- miRNA-mediated regulation during embryogenesis causes translational repression before mRNA decay

| PRJNA150397 | ENA

Danio rerio

Project description:Tris(1,3-dichloro-2-propyl) phosphate Exposure During Early-Blastula Alters the Normal Trajectory of Zebrafish Embryogenesis

| PRJNA475635 | ENA

Danio rerio

Project description:Genome-scale DNA methylation maps of Zebrafish Muller glia during retina regeneration

| PRJNA218577 | ENA

Danio rerio

Project description:Developmental features of DNA methylation during activation of the embryonic zebrafish genome

| PRJNA149411 | ENA

Danio rerio

Project description:Transcriptome Analysis of Zfpm1 Function in Early Zebrafish Embryogenesis and Postembryonic Heart Development