Project description:Whole-genome analysis of heat shock factor binding sites in Drosophila melanogaster. Heat shock factor IP DNA from non-shock (room temperature) Kc 167 cells compared to whole cell extract on Agilent 2x244k tiling arrays.
Project description:Whole-genome analysis of heat shock factor binding sites in Drosophila melanogaster. Heat shock factor IP DNA or Mock IP DNA from heat shocked Kc 167 cells compared to whole cell extract on Agilent 2x244k tiling arrays.
Project description:Whole-genome analysis of heat shock factor binding sites in Drosophila melanogaster using Heat Shock factor IP or Mock IP vs Whole cell Extract on Agilent 2x244k tiling arrays Heat Shock Factor IP or MOCK IP vs Whole Cell Extract
Project description:Mutants of the Mnn1 gene are hyper-sensitive to several stresses and display increased genome instability when subjected to conditions, such as heat shock, generally regarded as non-genotoxic. We describe a role for Menin as a global regulator of heat shock gene expression and critical factor in the maintenance of genome integrity 2-colour microarray design using 36 microarrays testing shock vs non shock in Drosophila melanogaster. Using a test of loss-of-heterozygosity, we show that Drosophila strains lacking a functional Mnn1 gene or expressing a Mnn1 dsRNAi are characterized by increased genome instability in response to short, repeated but non-lethal heat shock or hypoxia treatments. The same was true for strains lacking all Hsp70 genes.
Project description:Expression analysis of Heat Shock in Drosophila melanogaster KC167 Cells and 3rd instar dpcnbw larvae, and room temperature expression levels using Nimblegen arrrays (GPL8443) A 12 chip study using total RNA recovered from Heat shocked or non-heat shocked (room temperature) samples in either cells or larvae, 3 technical replicates for each treatment
