Dataset Information


Homo sapiens

ABSTRACT: Quantitative reduced representation bisulfite sequencing with unique molecular identifiers of single cell

ORGANISM(S): Homo sapiens  



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Q-RRBS: a quantitative reduced representation bisulfite sequencing method for single-cell methylome analyses.

Wang Kangli K   Li Xianfeng X   Dong Shanshan S   Liang Jialong J   Mao Fengbiao F   Zeng Cheng C   Wu Honghu H   Wu Jinyu J   Cai Wanshi W   Sun Zhong Sheng ZS  

Epigenetics 20150725 9

Reduced representation bisulfite sequencing (RRBS) is a powerful method of DNA methylome profiling that can be applied to single cells. However, no previous report has described how PCR-based duplication-induced artifacts affect the accuracy of this method when measuring DNA methylation levels. For quantifying the effects of duplication-induced artifacts on methylome profiling when using ultra-trace amounts of starting material, we developed a novel method, namely quantitative RRBS (Q-RRBS), in  ...[more]

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