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Genoma-wide analysis of pro- and anti-inflammatory activation states in embryonic stem cell-derived microglia

ABSTRACT: Genoma-wide analysis of pro- and anti-inflammatory activation states in embryonic stem cell-derived microglia

PROVIDER: PRJNA309379 | ENA |

REPOSITORIES: ENA

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Genoma-wide analysis of pro- and anti-inflammatory activation states in embryonic stem cell-derived microglia

Project description:Embryonic stem cell derived microglia (ESdM) were treated with different inflammatory stimulants to analyze their ability to adopt different activation states. Abstract: Microglia, the immune cells of the CNS, are highly adaptive cells that can acquire different pro- and anti-inflammatory activation states with distinct functions in CNS homeostasis and pathologies. To study microglial function in vitro, primary microglia or immortalized cell lines are commonly used. An alternative to these cells are embryonic stem cell-derived microglia (ESdM). ESdM have previously been shown to be very similar to primary microglia in terms of expression profiles and surface molecules. In this study, ESdM and primary microglia were treated with different inflammatory stimulants to analyze their ability to adopt different activation states. Using quantitative real time PCR, comparative transcriptomics, ELISA, and flow cytometry, we found that different activation states can be induced in ESdM, which are similar to those found in primary microglia. These states are characterized by specific sets of inflammatory marker molecules and differential transcriptome signatures. Our results show that ESdM are a valuable alternative cell model to study microglial functions and neuroinflammatory mechanisms.

2016-06-19 | GSE77062 | GEO

Characterization of pro- and anti-inflammatory activation states in embryonic stem cell derived microglia

Project description:Embryonic stem cell derived microglia (ESdM) were treated with different inflammatory stimulants to analyze their ability to adopt different activation states. These were characterized using ELISA, flow cytometry, quantitative real time PCR, and RNA-sequencing. Analysis of cytokine secretion, cell surface marker, gene expression, and RNA-seq expression data of differentially activated ESdM

2016-04-01 | E-GEOD-70383 | biostudies-arrayexpress

Genoma-wide analysis of pro- and anti-inflammatory activation states in primary microglia.

Project description:Murine primary microglia were treated with different inflammatory stimulants to induce different activation states and analyze the associated transcriptional changes. Abstract: Microglia, the immune cells of the CNS, are highly adaptive cells that can acquire different pro- and anti-inflammatory activation states with distinct functions in CNS homeostasis and pathologies. To study microglial function in vitro, primary microglia or immortalized cell lines are commonly used. An alternative to these cells are embryonic stem cell-derived microglia (ESdM). ESdM have previously been shown to be very similar to primary microglia in terms of expression profiles and surface molecules. In this study, ESdM and primary microglia were treated with different inflammatory stimulants to analyze their ability to adopt different activation states. Using quantitative real time PCR, comparative transcriptomics, ELISA, and flow cytometry, we found that different activation states can be induced in ESdM, which are similar to those found in primary microglia. These states are characterized by specific sets of inflammatory marker molecules and differential transcriptome signatures. Our results show that ESdM are a valuable alternative cell model to study microglial functions and neuroinflammatory mechanisms.

2016-06-19 | GSE77064 | GEO

Trem2 promotes anti-inflammatory responses in microglia and is suppressed under pro-inflammatory conditions

Project description:Trem2 promotes anti-inflammatory responses in microglia and is suppressed under pro-inflammatory conditions

| PRJNA663188 | ENA

Characterization of pro- and anti-inflammatory activation states in embryonic stem cell derived microglia

2016-04-01 | GSE70383 | GEO

Proteomic analysis of human microglia cells (HMC3) stimulated with anti-inflammatory cytokines using serum-enriched culture conditions

Project description:The immune functions of microglia in the brain are compromised in the presence of tumors that have the ability to “hijack” their pro-inflammatory nature by sending out signals that promote cancer progression. To analyze the tumor-supportive microglia phenotype, human microglia cells (HMC3) were treated with anti-inflammatory cytokines in serum-enriched cell culture media, and analyzed by LC-MS/MS using an Orbitrap QE instrument.

2021-10-06 | PXD023166 | Pride

Proteomic analysis of human microglia cells (HMC3) stimulated with anti-inflammatory cytokines using serum-deprived culture conditions

Project description:The immune functions of microglia in the brain are compromised in the presence of tumors that have the ability to “hijack” their pro-inflammatory nature by sending out signals that promote cancer progression. To analyze the tumor-supportive microglia phenotype, human microglia cells (HMC3) were treated with anti-inflammatory cytokines in serum-deprived cell culture media, and analyzed by LC-MS/MS using an Orbitrap QE instrument.

2021-10-07 | PXD023163 | Pride

Trem2 promotes anti-inflammatory responses in microglia and is suppressed under pro-inflammatory conditions

Project description:The purpose of this project was to investigate whole genome expression in primary microglia cultured from homozygous Trem2 R47H knock-in mice versus wild type littermates under basal conditions and in response to IL-4 treatment. The primary phenotype of microglia from Trem2 R47H knock-in mice was decreased expression levels of Trem2. IL-4 induced the expression of an anti-inflammatory programme of genes in microglia, which was attenuated when Trem2 expression was decreased.

2020-09-30 | GSE157891 | GEO

Anti-inflammatory effects of HDL in macrophages predominate over pro-inflammatory effects in atherosclerotic plaques

Project description:HDL infusion reduces atherosclerosis in animal models and is being evaluated as a treatment in humans. While some studies have shown anti-inflammatory effects of HDL in macrophages, others have reported pro-inflammatory effects and there is no consensus on underlying mechanisms. Transcriptional profiling reveals that HDL-mediated cholesterol efflux leads to both pro- and anti-inflammatory effects in LPS-stimulated macrophages. While early anti-inflammatory effects reflect reduced TLR4 levels, late anti-inflammatory effects are due to reduced interferon receptor signaling. Pro-inflammatory effects occur late and are ER stress responses mediated by IRE1a/ASK1/p38 MAPK signaling under conditions of marked cholesterol depletion. rHDL infusions in hypercholesterolemic atherosclerotic mice produced moderate anti-inflammatory effects in lesional macrophages without pro-inflammatory gene expression changes suggesting a beneficial therapeutic effect of HDL in vivo.

2020-04-04 | GSE129347 | GEO

Reprogramming of pro-inflammatory human macrophages to an anti-inflammatory phenotype by bile acids

Project description:Reprogramming of pro-inflammatory human macrophages to an anti-inflammatory phenotype by bile acids

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