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PfAP2-I-GFP-msp5MUT vs PfAP2-I-GFP 3D7

ABSTRACT: PfAP2-I-GFP-msp5MUT vs PfAP2-I-GFP 3D7

PROVIDER: PRJNA311596 | ENA |

REPOSITORIES: ENA

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PfAP2-I-GFP-msp5MUT vs PfAP2-I-GFP 3D7 [timecourse 2]

Project description:Obligate intracellular parasites must efficiently invade host cells in order to mature and be transmitted. For the malaria parasite Plasmodium falciparum, invasion of host red blood cells (RBCs) is essential. Here we describe a parasite-specific transcription factor belonging to the Apicomplexan Apetala 2 (ApiAP2) family that is responsible for regulating the expression of a subset of merozoite genes involved in RBC invasion (PfAP2-I). Our genome-wide analysis by ChIP-seq shows that PfAP2-I interacts with a specific DNA motif in the promoters of these genes. msp5 transcription levels decrease when the PfAP2-I DNA-binding motif is mutated in PfAP2-I-GFP parasites, showing that PfAP2-I must bind the DNA motif in order for msp5 to be transcribed.

2016-12-31 | GSE77809 | GEO

PfAP2-I-GFP-msp5MUT vs PfAP2-I-GFP 3D7 [timecourse 1]

2016-12-31 | GSE77807 | GEO

Transgenic Plasmodium falciparum parasites line 3D7/DDGFP-PfAP2-HC: transcriptional differences between 3D7/DDGFP-PfAP2-HC control parasites (cultured in presence of Shield-1; ON) vs PfAP2-HC-depleted parasites (cultured in absence of Shield-1; OFF)

Project description:Transcriptional profiling of transgenic P. falciparum asexual blood stage parasites of the transgenic strain 3D7/DDGFP-PfAP2-HC at five time points during intra-erythrocytic parasite development. The DD (FKBP destabilisation domain) allows for the conditional expression of fusion proteins: DD fusion proteins are rapidly degraded or stably expressed in absence or presence of the stabilising ligand Shield-1, respectively (Banaszynski LA, Chen LC, Maynard-Smith LA, Ooi AG, Wandless TJ. A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules.Cell. 2006 Sep 8;126(5):995-1004). The goal of this experiment was to identify genes differentially expressed in DDGFP-PfAP2-HC-expressing compared to DDGFP-PfAP2-HC-depleted parasites during the intra-erythtrocytic cell cycle.

2020-10-06 | GSE159061 | GEO

PfAP2-I-GFP-msp5MUT vs PfAP2-I-GFP 3D7

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-12-31 | GSE77810 | GEO

Plasmodium falciparum

Project description:PfAP2-I-GFP-msp5MUT vs PfAP2-I-GFP 3D7 [timecourse 2]

| PRJNA311599 | ENA

Plasmodium falciparum

Project description:PfAP2-I-GFP-msp5MUT vs PfAP2-I-GFP 3D7 [timecourse 1]

| PRJNA311602 | ENA

RNAseq of GFP-sorted cells (Low-GFP vs. High-GFP, GFP-fed vs GFP+fed)

Project description:RNAseq of GFP-sorted cells (Low-GFP vs. High-GFP, GFP-fed vs GFP+fed)

| PRJNA544322 | ENA

PfAP2-I-GFP anti-GFP and anti-IgG ChIP-seq

Project description:To determine the genome-wide occupancy of the Plasmodium falciparum transcriptional regulator of invasion PfAP2-I (PfDd2_100013100/PF3D7_1007700), we used chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq). Synchronized, schizont stage, 40 hours post-invasion, cultures of parasites expressing the AP2-I-GFP fusion protein were treated with formaldehyde to crosslink proteins to DNA and harvested. After shearing the DNA, the chromatin was incubated with anti-GFP antibody or IgG (as control) for immunoprecipitation. This material was used to generate Illumina sequencing libraries. The final libraries were multiplexed with fourteen barcoded samples per lane on an Illumina HiSeq 2500 system to generate 150 base pair single-end reads.

2017-06-01 | GSE80293 | GEO

P. falciparum (lab strain 3d7) schizonts vs. P. falciparum (lab strain 3d7) reference RNA pool

Project description:This SuperSeries is composed of the following subset Series: GSE33795: P. falciparum (lab strain 3d7) schizonts untreated control vs P. falciparum (lab strain 3d7) reference RNA pool GSE33796: P. falciparum (lab strain 3d7) schizonts treated with ionomycin vs P. falciparum (lab strain 3d7) reference RNA pool GSE33797: P. falciparum (lab strain 3d7) schizonts treated with A23187 vs P. falciparum (lab strain 3d7) reference RNA pool Refer to individual Series

2012-07-14 | E-GEOD-33868 | biostudies-arrayexpress

extracellular calcium chelation experiment_P. falciparum (lab strain 3d7) schizonts vs P. falciparum (lab strain 3d7) reference RNA pool

Project description:This SuperSeries is composed of the following subset Series: GSE33834: extracellular calcium chelation experiment_P. falciparum (lab strain 3d7) schizonts untreated vs P. falciparum (lab strain 3d7) reference RNA pool GSE33835: extracellular calcium chelation experiment_P. falciparum (lab strain 3d7) schizonts treated with ionomycin vs P. falciparum (lab strain 3d7) reference RNA pool GSE33836: extracellular calcium chelation experiment_P. falciparum (lab strain 3d7) schizonts treated with EGTA and ionomycin vs P. falciparum (lab strain 3d7) reference RNA pool Refer to individual Series

2012-07-14 | E-GEOD-33869 | biostudies-arrayexpress

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