Project description:In this study, we use mRNA-Seq to characterize and compare the leukocyte transcriptomes of Holstein, Jersey, and Cholistani with respect to variations in sequence, expression, and splicing. Poly A+ RNA are extracted from leukocytes of pools of blood drawn from cows of three different breeds, Holstein, Jersey, and Cholistani. The objective of the study is to compare sequence variation and gene expression of the three cattle breeds.
Project description:The objective of this study was to characterize the mRNA expression profile in rumen epithelium from Holstein dairy cows fed high or low concentrate dits.
Project description:We investigated changes in rumen fermentation, peripheral blood metabolites and hormones, and hepatic transcriptomic dynamics in Holstein cows with and those without subacute ruminal acidosis (SARA) during the periparturient period.
Project description:SARST-V1 method was used to asses the effect of live yeast on the microbial population of the rumen of cows fed an acidogenic diet 3 cows were used in 3 by 3 latin-square design with 3 periods. In each period animals received either 0.5g/d of yeast, 5g/d of yeast or none. Rumen microbiota was analysed using the SARST-V1 method for each period.
