Project description:We compared the transcriptomic differences in rice leaves after being fed by C. medinalis using RNA sequencing. Global transcriptome changes in rice leaves responding to 1, 6, 12 and 24 h of such feeding were quantified by examining mRNAs of 20 libraries (four biological replicates at each of five sampling times) that collectively included 164.04 Gb of clean reads. Each of these samples contained at least 7.36 Gb of data with Q30 quality scores 90.70%. The GC content accounted for 53.16-53.71% of these reads. A total of 44.84-56.19 million reads were mapped uniquely to the rice IRGSP-1.0 reference genome, with unique mapping rates ranging from 89.73% to 91.36%. Using absolute value of log2(fold change) > 0 and FDR adjusted P value (padj) ≤ 0.05 as threshold for recognizing differentially expressed genes between any two treatments, a total of 6,372 DEGs were identified in rice leaves at different time points (1, 6, 12, and 24 h) of C. medinalis infestation.
