Project description:Solventogenic Clostridium species ferment carbohydrates to acetone, butanol and ethanol which are well-known next-generation biofuels. However, repeated subculture of or continuous fermentation by Clostridium often decreases and eventually terminates the solvent production and spore formation, which is a process called strain degeneration. Supplementation of CaCO3 to fermentation medium could partially recover metabolism of degenerated strain by more than 50% increase of cell growth and solvent production. The transcriptome profile of Clostridium beijerinckii NCIMB 8052 (DG-8052) and its response to CaCO3 treatment were analysed by microarray. Since fermentation by C. beijerinckii NCIMB 8052 is a biphasic process, gene expressions of two fermentations were compared at each stage, i.e. 12h and 24h fermentation time representing acidogenic phase and solventogenic phase, respectively. This study examined expression of 5168 genes capturing 98.6% of the C. beijerinckii NCIMB 8052 genome. With the addition of CaCO3, DG-8052 had 565 and 916 genes significantly up-regulated at acidogenic phase and solventogenic phase, respectively. According to the enrichment analysis of pathway and Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, these genes were significantly overrepresented in cellular functions such as Amino acid transport and metabolism, organic acid biosynthetic process, bacteria chemotaxis and defense mechanisms. On the other hand, there were 704 and 1044 genes significantly down-regulated at acidogenic phase and solventogenic phase, respectively. These repressed genes were mainly enriched in functions such as ion transmembrane transport, ATP synthesis, oxidative phosphorylation.

2017-01-02 | GSE81808 | GEO

Methylomonas rhizoryzae strain:GJ1

Project description:Methylomonas rhizoryzae strain:GJ1 Genome sequencing and assembly

| PRJNA564367 | ENA

Methylomonas koyamae strain:LM6

Project description:Methylomonas koyamae strain:LM6 Genome sequencing and assembly

| PRJNA394621 | ENA

Determination of WatR binding site in Methylomonas sp. DH-1 after lactate adaptive evolution

Project description:JHM80 strain was obtained by adaptive evolution of Methylomonas sp. DH-1 strain against 8.0 g/L of lactate. The tolerance against lactate of JHM80 was obtained by the overexpression of watR (weak acid tolerance regulator). In order to discover the target genes of WatR, FLAG epitope tag was added to WatR of JHM80 strain and ChIP-seq was performed.

2023-08-08 | GSE206216 | GEO

Methylomonas paludis strain:S2AM

Project description:Methylomonas paludis strain:S2AM Genome sequencing

| PRJNA724036 | ENA

Methylomonas denitrificans strain:FJG1

Project description:Methylomonas denitrificans strain:FJG1 Genome sequencing

| PRJNA258394 | ENA