Project description:To reveal genome-wide direct targets of AtbZIP17, we carried out Chromatin immuno-precipitation coupled with high-throughput sequencing (ChIP-seq) experiments with heat-stressed MYC-bZIP17 expressing seedling plants. For ChIP-seq, 13-day-old 35Spro::MYC-bZIP17 seedlings grown at 22°C were placed at 42°C for 2 h.
Project description:The NGS-associated mRNA-seq analysis was conducted to survey transcriptome changes responding to three UPR inducers (tunicamycin, DTT, & Azetidine-2-cytosine) by four double mutant of three UPR-associated transcription factors (bZIP17, bZIP28, & bZIP60) and two activators (S1P & S2P).
