Project description:Purpose: The present study provides the firstly large-scale characterization of miRNAs in Tetranychus cinnabarinus and the comparison between fenpropathrin resistant and susceptible strains gives a clue on study how miRNA involving in fenpropathrin resistance Methods: Using Illumina sequencing to identify the differentially expressed miRNAs between the fenpropathrin resistant and susceptible strains of Tetranychus cinnabarinus Results: 12 miRNAs that were expressed significantly differently were identified between thethe fenpropathrin resistant and susceptible strains of Tetranychus cinnabarinus Total RNA obtained from 500 female adults aged 3-5 days post emergence from fenpropathrin resistant and susceptible strains of Tetranychus cinnabarinus,two biological repeats for each strain Raw sequencing read data were submitted to SRA: SRP067789
Project description:Purpose: The present study provides the firstly large-scale characterization of miRNAs in Tetranychus cinnabarinus and the comparison between fenpropathrin resistant and susceptible strains gives a clue on study how miRNA involving in fenpropathrin resistance Methods: Using Illumina sequencing to identify the differentially expressed miRNAs between the fenpropathrin resistant and susceptible strains of Tetranychus cinnabarinus Results: 12 miRNAs that were expressed significantly differently were identified between thethe fenpropathrin resistant and susceptible strains of Tetranychus cinnabarinus
Project description:RNA-seq was employed to analyze the transcriptome changes in T. cinnabarinus treated with curcumin or solvent. A total of 105, 706, 297 clean sequence reads were generated from the sequencing with more than 90% of the reads were successfully mapped to the reference sequence. The RNA-seq identified 111 and 96 differentially expressed genes between curcumin and solvent treated mites at 24 h and 48 h post treatment, respectively. GO enrichment analysis showed that differentially expressed genes from 24 h post treatment were categorized into 35 GO functional groups and differentially expressed genes from 48 h post treatment were categorized into 25 GO functional groups. The cellular process under the category of biological process was dominant throughout the GO classification at both time points. Finally, we screened 23 differentially expressed genes that are functionally identical or similar to the targets of common insecticide/acaricides or associated with mite detoxification and metabolism. Overall our results revealed the global transcriptional changes in T. cinnabarinus upon curcumin treatment and will enable the further identification of the targets of curcumin on mite.
