Project description:To overview compound-responsive genes in fibroblast, we performed microarray analysis of ~63,000 genes in compound-treated fibroblast of LSND3 Overall design: We treated the fibroblasts with 1μM compound, extracted RNAs from compound- and non-treated fibroblasts at 24 h after treatment, and compared the gene expression ratio between the two groups. Two duplicate experiments.
Project description:The virulon of Staphyloccocus aureus is controlled by intricate connections between transcriptional and post-transcriptional regulators including proteins and small non coding RNAs (sRNAs). Most of the sRNAs regulate gene expression through base-pairings with mRNAs. Here, we have determined the transcriptome of RsaI sRNA of S. aureus. Overall design: Global overview of RsaI impact on gene regulation, a comparative transcriptomic analysis was performed on total RNAs extracted from the WT HG001 strain, the isogenic HG001∆rsaI mutant strain, and the same mutant strain complemented with a plasmid expressing RsaI under the control of its own promoter
Project description:To obtain an overview of the Oviductus Ranae gene expression profile during hibernation, a cDNA sample was prepared from Oviductus Ranae and sequenced using the Illumina sequencing platform.
Project description:To obtain an overview of the antler tip gene expression profile during the ossification stage, a cDNA sample was prepared from antler tip and sequenced using the Illumina sequencing platform.
Project description:To obtain an overview of the Oviductus Ranae gene expression profile during initial growing period, a cDNA sample was prepared from Oviductus Ranae and sequenced using the Illumina sequencing platform
Project description:To obtain an overview of the antler tip gene expression profile during rapid growth period, a cDNA sample was prepared from antler tip and sequenced using the Illumina sequencing platform.
Project description:We provide a broad overview of sequence diversity in An. gambiae mature microRNAs, including annotation of novel microRNAs identified in this study. Non-coding RNA profiling by high throughput sequencing, in duplicate, using Illumina GAIIx.
Project description:The present study show the impact of macrophages activation by IFN-Gamma on the gene regulation in intracellularly replicating L. monocytogenes and provide an overview about additionally adaption mechanisms of L. monocytogenes to immune response.
Project description:Goal:obtaing an overview of gene expression changes in BxPC3 cells rendered deficient in beta-catenin protein due to a zinc-finger nuclease mediated disruption of the beta-catenin gene (CTNNB1). Overall design: Total RNA from exponentially growing cells were harvested and subjected to further analysis
Project description:The method DFI-seq was developed to enable identification of differentially expressed genes in uropathogenic E. coli strain UTI89 during growth in human urine and in bladder epithelial cells. By utilising this new method, the aim was to identify novel virulence genes in UTI89. DFI-seq is a combination of differential fluorescence induction (DFI) with next-generation sequencing. DFI-seq was compared to DFI by analysing gene expression of UPEC in human urine and thereby confirming that DFI-seq gives a better overview of gene expression. DFI-seq was hereafter used to look at gene expression in UTI89 while infecting bladder epithelial cells. We demonstrate the usefulness of DFI-seq for identification of genes required for optimal growth of UPEC in human urine, as well as potential virulence genes upregulated during infection of bladder epithelial cells. DFI-seq holds potential for the study of bacterial gene expression in live-animal infection systems.