Project description:We show that Herpes simplex virus 1 (HSV-1) induces the expression of about 1000 antisense transcripts from the human host cell genome. Overall design: Human WI-38 and HeLa cells were infected with Herpes simplex virus 1, and antisense transcripts on the host cell genome defined using a custom algorithm.
Project description:Comparison of whole genome exome array CGH to a commercial SNP array for detection of de novo and homozygous copy number variants in 99 autism simplex trios. Will update once manuscript is prepared. Overall design: Agilent custom designed array comparative genomic hybridization (CGH) was performed on 100 samples with a diagnosis of autism. Unaffected parents of the 100 probands were also tested. A common male reference was used for all samples and parents. Samples were obtained from the Simons Simplex Collection. Three samples (11154_P1, 11154_Mo, 11154_Fa) were removed from the final analysis for quality control.
Project description:We introduce here SIMPLEX (SImultaneous Metabolite, Protein, Lipid EXtraction procedure), a unique strategy for the quantitative investigation of lipids, metabolites and proteins. SIMPLEX offers a fundamental turn in study design since multimolecular classes can be accessed in parallel from one sample, with equal efficiency and reproducibility compared to unimolecular workflows.