Project description:Expression data from two weeks old Arabidopsis wild-type (Nössen:NO) and at4g16790 (here renamed DUF761-1) knock-out mutant RATM11-0975-1_H (here named duf761-1); Expression data from two weeks old Arabidopsis wild-type (Columbia-0) and transgenic plant overexpressing DUF761-1 (at4g16790) plants(here named OE6) Microarray assays can facilitate elucidation of cellular processes and gene network functions in the processes of plant growth and development. To gain further insight into the potential molecular role of the at4g16790 gene (here named DUF761-1), we performed Affymetrix whole-genome microarray analysis (http://www.affymetrix.com/) on Nössen and duf761-1plants, and on Columbia-0 and OE6 plants, to compare their genome-wide expression profiles under 22 °C. The differently expressed genes revealed by transcriptional profiling indicate that DUF761-1 may involve in plant cell wall biology and defense response of Arabidopsis.
Project description:We have used Agilent whole genome arrays (v4) to compare the gene expression changes between 5 days-old Arabidopsis ga1 mutants, pif1345 mutants (Leivar et al., 2008) and transgenic lines overexpressing the regulators GNC and GNL/CGA1 to the wild type. All mutants or transgenic lines are in the Columbia-0 background. Three independent biological samples were prepared for each genotype.
Project description:Transcriptional profiling of Arabidopsis rossette leaves comparing WT Col-0 with a transgenic line overexpressing AhERF or AhDOF genes from Amaranthus hypochondriacus under different conditions. Three-condition experiment of WT vs AhERF OE plant leaves. The analyzed conditions were: normal growth conditions, 5 days of water stress (no irrigation) and 24 hrs of recovery after watering water-stressed plants. Besides, a two-condition experiment where WT vs AhDOF OE plant leaves were compared. The experimental conditions were: normal growth conditions and plants watered with 40mL of 400mM NaCl solution for three straight days to produce salt stress.
Project description:Identification of target genes for the transcription factor HSFA4A in Arabidopsis thaliana. Two weeks-old Col-0 wild-type and transgenic plants overexpressing the HSFA4A transcription factor under the estradiol-inducible promoter lexA (HSFA4Aox2) were treated by 5µM estradiol with or without 1mM H2O2 for 6h in liquid 0.5 MS medium and the isolated RNA samples were used for RNAseq analysis.
Project description:This study analyzes transcriptomic data of Arabidopsis thaliana Col-0 and overexpression lines of Hypoxia Response Attenuator (HRA1; At3g10040) with Col-0 background (OE-HRA1). Two independent transgenic lines of OE-HRA1 were considered as biological replicates (OE-HRA1#1 and OE-HRA1#2). Seven-day-old seedlings were treated either with or without hypoxia (low oxygen) stress for 2 hours. This dataset includes CEL files, RMA signal values and MAS5 P/M/A calls from total mRNA populations. Quantitative profiling of cellular mRNAs was accomplished with the Affymetrix ATH1 platform.
Project description:Microarray assays can facilitate elucidation of cellular processes and gene network functions in the processes of plant growth and development. To gain further insight into the potential molecular role of the NHRGP gene, we performed Affymetrix whole-genome microarray analysis (http://www.affymetrix.com/) on WT and OE-N6 plants to compare their genome-wide expression profiles. The differently expressed genes revealed by transcriptional profiling of an HRGP-overexpressing transgenic plant indicate that NHRGP are involved in defense response.
Project description:Arabidopsis Affymetrix ATH1 GeneChips were used to compare the mRNA profiles of root tissues of the transgenic plants overexpressing 4D09 effector gene from the cyst nematode Heterodera schachtii and the wild-type (C24). Also, Arabidopsis Affymetrix ATH1 GeneChips were used to compare the mRNA profiles of root tissues of the transgenic plants overexpressing 14-3-3Ɛ gene from Arabidopsis and the wild-type (Col-0). Wild-type (Arabidopsis thaliana, ecotypes C24 and Col-0 ), and the transgenic plants overexpressing 4D09 effector gene or overexpressing 14-3-3Ɛ gene from Arabidopsis were grown in vertical culture dishes on modified Knop’s medium for 2 weeks and then root tissues were collected for RNA extraction. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Tarek Hewezi. The equivalent experiment is AT144 at PLEXdb.]
Project description:Two independent Solanum tuberosum ssp. Andigena ADG StCEN RNAi and two ADG StCEN 35S overexpressing OE transgenic lines and a wild type WT control were grown under standard glasshouse conditions for 6 weeks prior to being moved to controlled growth cabinet conditions. Plants were grown under 4 different daylengths (8,10,12 and 16 hr) at 20 °C day and 14 °C night temperatures. ADG StCEN RNAi lines showed signs of early tuberization phenotype compared with the WT control after 23 days in the cabinets. Non-swelling stolons were harvested at this timepoint. ADG StCEN 35S OE lines demonstrated a delayed tuberization phenotype compared with the WT control. Non-swelling stolons were harvested after 38 days in the controlled cabinet conditions.
Project description:Transcriptional profiling of Arabidopsis rossette leaves comparing WT Col-0 with a transgenic lines overexpressing AhNF-YC gene from Amaranthus hypochondriacus in three different conditions. Three-condition experiment WT vs AhNF-YC OE plant leaves. The analyzed conditions were: normal growth conditions, water stress for 5 days and 24 hrs of recovery after normal watering was reestablished to 8-day water stressed plants.
Project description:In order to investigate the possible molecular mechanisms of SlHsfA3 function in plant growth and stress responses, we employed the RNA-seq approach to identify the genes with altered expression levels in the transgenic Arabidopsis overexpressing SlHsfA3. Processing of RNA samples on the Illumina HiSeq 2000 system yielded more than 24 million reads, each 100bp in length, encompassing 2.4Gb of sequence data for each sample which was then mapped to the reference genome. Quantitative analysis of RNA-seq data identified substantial variation in expression profiles among different genotypes, consistent with known functional differences. Transcript abundance obtained from RNA-seq data was indicated as RPKMs and therefore, Up- and down-regulated genes were determined by a greater than 2-fold change of normalized RPKMs in comparison analysis (35S:SlHsfA3 transgenic plants versus Col-0(WT) plants) in both over-expression lines: Q-value < 0.05. The statistical analysis identified a total of 181 differentially expressed genes between OE lines and Col-0(WT) at Q-value < 0.05, among which 114 (63%) were up-regulated and 67 (37%) were down-regulated with fold changes higher than 2. RNA-sequencing was carried out using two independent transgenic lines (35S:SlHsfA3-3 and 35S:SlHsfA3-6). Total RNA was isolated with Trizol reagent (Invitrogen, USA) from the aerial part of the 4-week-old seedlings of 35Spro:SlHsfA3 and Col-0 (WT) plants grown in parallel under unstressed conditions. Materials from 20 plants of each genotype were pooled for RNA isolation.
