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Omics score: 1000

Actinosynnema pretiosum strain:ATCC 31280

ABSTRACT: Actinosynnema pretiosum strain:ATCC 31280 Genome sequencing and assembly

PROVIDER: PRJNA323403 | ENA |

REPOSITORIES: ENA

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Genome-Scale Metabolic Model of Actinosynnema pretiosum ATCC 31280 and Its Application for Ansamitocin P-3 Production Improvement.

Project description:Actinosynnema pretiosum ATCC 31280 is the producer of antitumor agent ansamitocin P-3 (AP-3). Understanding of the AP-3 biosynthetic pathway and the whole metabolic network in A. pretiosum is important for the improvement of AP-3 titer. In this study, we reconstructed the first complete Genome-Scale Metabolic Model (GSMM) Aspm1282 for A. pretiosum ATCC 31280 based on the newly sequenced genome, with 87% reactions having definite functional annotation. The model has been validated by effectively predicting growth and the key genes for AP-3 biosynthesis. Then we built condition-specific models for an AP-3 high-yield mutant NXJ-24 by integrating Aspm1282 model with time-course transcriptome data. The changes of flux distribution reflect the metabolic shift from growth-related pathway to secondary metabolism pathway since the second day of cultivation. The AP-3 and methionine metabolisms were both enriched in active flux for the last two days, which uncovered the relationships among cell growth, activation of methionine metabolism, and the biosynthesis of AP-3. Furthermore, we identified four combinatorial gene modifications for overproducing AP-3 by in silico strain design, which improved the theoretical flux of AP-3 biosynthesis from 0.201 to 0.372 mmol/gDW/h. Upregulation of methionine metabolic pathway is a potential strategy to improve the production of AP-3.

| S-EPMC6070911 | BioStudies

Transcriptome of ansamitocin P-3 producer Actinosynnema pretiosum ATCC 31280

Project description:In submerged cultivation of filamentous microbes, including actinomycetes, complex morphology is one of the critical process features for secondary metabolites production. Ansamitocin P-3 (AP-3), an antitumor agent, is a secondary metabolite produced by Actinosynnema pretiosum ATCC 31280. An excessive mycelial fragmentation of A. pretiosum ATCC 31280 was observed during the early stage of fermentation. In order to identify genes involved in the early mycelial fragmentation, the total RNAs of mycelia collected at 15, 18, and 24 h were extracted and subjected to transcriptome sequencing using RNA-seq technology.Through comparative transcriptomic analysis, a subtilisin-like serine peptidase encoded gene APASM_4178 was identified to be responsible for the mycelial fragmentation. Mutant WYT-5 with the APASM_4178 deletion showed increased biomass and improved AP-3 yield by 43.65%. Overall design: Exploration of the expression profile of genes in different mycelial morphology and search for mycelial fragmentation related genes. Three samples are analyzed.

2020-05-25 | GSE151010 | GEO

Genome-Guided Discovery of Pretilactam from Actinosynnema pretiosum ATCC 31565.

Project description:Actinosynnema is a small but well-known genus of actinomycetes for production of ansamitocin, the payload component of antibody-drug conjugates against cancers. However, the secondary metabolite production profile of Actinosynnema pretiosum ATCC 31565, the most famous producer of ansamitocin, has never been fully explored. Our antiSMASH analysis of the genomic DNA of Actinosynnema pretiosum ATCC 31565 revealed a NRPS-PKS gene cluster for polyene macrolactam. The gene cluster is very similar to gene clusters for mirilactam and salinilactam, two 26-membered polyene macrolactams from Actinosynnema mirum and Salinispora tropica, respectively. Guided by this bioinformatics prediction, we characterized a novel 26-membered polyene macrolactam from Actinosynnema pretiosum ATCC 31565 and designated it pretilactam. The structure of pretilactam was elucidated by a comprehensive analysis of HRMS, 1D and 2D-NMR, with absolute configuration of chiral carbons predicted bioinformatically. Pretilactam features a dihydroxy tetrahydropyran moiety, and has a hexaene unit and a diene unit as its polyene system. A preliminary antibacterial assay indicated that pretilactam is inactive against Bacillus subtilis and Candida albicans.

| S-EPMC6631532 | BioStudies

Actinosynnema pretiosum subsp. pretiosum strain:ATCC 31280

Project description:Actinosynnema pretiosum subsp. pretiosum strain:ATCC 31280 Genome sequencing

| PRJNA722727 | ENA

Actinosynnema pretiosum subsp. pretiosum strain:ATCC 31280

Project description:Actinosynnema pretiosum subsp. pretiosum strain:ATCC 31280 Genome sequencing and assembly

| PRJNA471980 | ENA

Actinosynnema pretiosum subsp. pretiosum strain:ATCC 31280

Project description:Actinosynnema pretiosum subsp. pretiosum strain ATCC 31280 ansamitocin gene cluster I

| PRJNA361200 | ENA

Actinosynnema pretiosum subsp. pretiosum strain:ATCC 31280

Project description:Actinosynnema pretiosum subsp. pretiosum strain ATCC 31280 ansamitocin gene cluster II

| PRJNA362988 | ENA

Transcriptome of the ansamitocin producer Actinosynnema pretiosum ATCC 31280

Project description:Transcriptome of the ansamitocin producer Actinosynnema pretiosum ATCC 31280

| PRJNA475541 | ENA

Transcriptome of ansamitocin P-3 producer Actinosynnema pretiosum ATCC 31280

Project description:Transcriptome of ansamitocin P-3 producer Actinosynnema pretiosum ATCC 31280

| PRJNA634364 | ENA

Transcriptome of Actinosynnema pretiosum ATCC 31280 under exogenous supplement of AP-3

Project description:In search of AP-3 resistant genes, ATCC 31280△ansaA (inactivation of ansamitocin pksA, in avoiding of the interruption of endogenous generated AP-3) were cultured under the addition of 0, 50, 100 and 200 mg/L exogenous AP-3 for 24 h and collected, for strains under 200 mg/L AP-3, samples after 48 h and 72 h were also collected, and the RNA samples were collected and sent for sequencing. Overall design: Exploration of the expression profile of genes under exogenous addition of AP-3 and search for ansamitocin resistant genes.

2019-11-06 | GSE139930 | GEO

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