A novel marine strain representing efficient degradation ability toward brown algae was isolated, identified, and assigned to Bacillus weihaiensis Alg07. The alga-associated marine bacteria promote the nutrient cycle and perform important functions in the marine ecosystem. The de novo sequencing of the B. weihaiensis Alg07 genome was carried out. Results of gene annotation and carbohydrate-active enzyme analysis showed that the strain harbored enzymes that can completely degrade alginate and lam ...[more]
Project description:Investigation of whole genome gene expression level changes in sporulating Bacillus subtilis 168 delta-prpE mutant, compared to the wild-type strain. The mutation engineered into this strain results in impaired germination of spores. A six chip study using total RNA extracted from three separate wild-type cultures of sporulating Bacillus subtilis 168 and three separate cultures of sporulating mutant strain, Bacillus subtilis 168 delta-prpE, in which prpE (yjbP BSU11630) gene coding for a protein phosphatase is deleted entirely. Each chip consists of four fields able to measure the expression level of 4,104 genes from Bacillus subtilis subsp. subtilis strain 168 NC_000964 with eight 60-mer probe pairs (PM/MM) per gene, with two-fold technical redundancy.
Project description:Comparative Genomic Hybridization. Analysis of genomic content of closely related Bacillus species. Refer to individual records for strain information. Refer to platform and individual sample records for experimental protocols.
Project description:Transcriptional profiling of C. elegans young adult worms cultured on non-pathogenic Bacillus strain 67 versus versus age-matched worms cultured on the control lab food E. coli OP50. The goal was to identify genes regulated in response to differences in diet, which might confer immunity to later exposures to pathogenic Bacillus thuringiensis DB27. One-condition experiment. C. elegans young adults, cultured on : Bacillus strain 67 versus E. coli OP50. 4 biological replicates, including 2 dye-swaps.
Project description:Investigation of whole genome expression level changes in Bacillus anthracis Sterne deltaClpX mutant compared to the wild-type strain after growth in nutrient rich media. The deltaClpX mutant used in this study is described in McGillivray et al. 2009. ClpX Protease Contributes to Antimicrobial Peptide Resistance and Virulence Phenotypes of Bacillus anthracis. Journal of Innate Immunity 1(5): 494-506. Overall design: A six chip study using total RNA recovered from three separate cultures of the wild-type Bacillus anthracis Sterne strain and three separate cultures of the deltaClpX mutant strain. Each chip measures the expression level of 5287 chromosomal genes from Bacillus anthracis Sterne.
Project description:To determine Sigma 54 (SigL) reglons in Bacillus thuringiensis HD73 strain, A sigLmutant, HD(ΔsigL::kan), was constructed with insertion of kanamycin resistance gene cassete. We have employed whole genome microarray expression profiling as a discovery platform to identify the difference of gene expression between mutant and wild-type strains. 2 ml samples were separately harvested from B. thuringiensis HD73 and HD(ΔsigL::kan) strains grown in Schaeffer’s sporulation medium (SSM) at stages T7 of stationary phase (7 hours after the end of the exponential phase). Three independent repeats were performed for each stain.
Project description:The aim of the study was to carry out a CGH study utilizing a set of 39 diverse Bacillus isolates. Thirty four B. cereus and five B. anthracis strains and isolates were chosen so as to represent different lineages based on previous characterizations, including MLEE and MLST (Helgason, Okstad et al. 2000; Helgason, Tourasse et al. 2004). They represent the spectrum of B. cereus phenotypic diversity by including soil, dairy and periodontal isolates in addition to virulent B. anthracis strains. Overall design: 39 diverse Bacillus isolates were chosen for the study.Thirty four B. cereus and five B. anthracis strains. Dye swap experiments were performed yielding 2 hybridizations per query strain. Each 70mer oligo spotted on the B. cereus species microarray is spotted once. Positive controls on the array consist of oligos designed from the sequenced reference genome, STERNE, and negative controls on the array consist of oligos designed from the thale cress plant, Arabidopsis thaliana.
Project description:Comparison at t2 (two hours into post-exponential phase growth as analyzed by OD measurements) of global expression profiles from a Bacillus thuringiensis 407 delta-sinI delta-sinR double gene deletion strain versus a Bacillus thuringiensis 407 delta-sinI single gene deletion strain, to analyze global expression changes following deletion of the sinR transcriptional regulator gene in a sinI-negative background.
Project description:This series represents the work described in the publication Bacillus subtilis Genome Diversity by Earl et al. (Journal of Bacteriology, accepted) Keywords: comparative genomic hybridization Overall design: The aim of this study was to examine genome diversity among Bacillus subtilis species members. Strains were chosen from within both recognized subspecies and one close relative, Bacillus vallismortis.