Jatropha curcas
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PROVIDER: PRJNA32385 | ENA |
REPOSITORIES: ENA
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Project description:Casbene synthase is responsible for the first commited step in the biosynthesis of phorbol esters (PE) in the Euphorbiaceae. PE are abundant in the seeds of the biofuel crop Jatropha curcas and its toxicity precludes the use of the protein-rich cake obtained after oil extraction as an animal feed and the toxicity of the fumes derived from burning PE containing biofuel is also a matter of concern. This toxicity is a major hindrance to exploit the potential of this crop as a source of raw material for the production of biodiesel. For this reason, current research on J. curcas is mainly focused in the understanding of the biosynthesis and site of synthesis of PE, as an avenue for the development of biotechnological of genotypes unable to synthesize PE in its seeds. Here, we present the results of targeted proteome assays (SRM and PRM) to monitor and quantify casbene synthase in leaves, endosperma and roots of two J. curcas of contrasting levels of PE. The assays were based in the use of synthetic isotopic labeled peptides derived from 12 gene models of casbene synthase from the J. curcas genome and results showed the presence of casbene synthase encoded in seven of the 12 gene models. Several specific transitions were identified and which can be used to monitor several casbene synthase proteins by any one of the two targeted proteomics assays and/or to validate the results of transcription-based experiments.
2021-09-09 | PXD019525 | Pride
Project description:Jatropha is known for its ability to grow in marginal lands and drought prone areas receiving limited amounts of rainfall. Accordingly, gene discovery in Jatropha will be useful for providing a source of genetic information for the improvement of drought tolerance in crops. In this study, a Jatropha oligomicroarray was developed to evaluate the gene expression profile of Jatropha plants during drought stress response and recovery from stress. When the gene expression patterns were compared between those differentially expressed during exposure to drought stress and re-watering, it was possible to identify 333 genes that are involved in the response to dehydration, while 592 genes were found to be significant during recovery, and 375 genes are associated in both dehydration and recovery. Furthermore, representative genes from the three gene categories were compared to those found in other plant species and a basic understanding on how Jatropha copes with drought and its mechanism for survival in dry conditions is discussed. Taken together, the oligomicroarray that we developed in this study is a useful tool for analyzing expression profiles of Jatropha genes to better understand molecular mechanism underlying drought stress responses as well as other aspects of molecular studies in Jatropha.
2014-11-30 | GSE55847 | GEO
Project description:Jatropha curcas, a tropical shrub, is an attractive biofuel crop, which produces seeds with a high content of oil and protein. Most J. curcas accessions contain a range of toxins and anti-nutritional compounds, which are unfavorable for human consumption and animal feed. With the goal of better understanding the development of its seeds and to improve Jatropha`s agronomic performance, a two-step approach was performed: 1) generation of the entire transcriptome of six different developmental stages of J. curcas seeds using 454-Roche sequencing of a cDNA library, 2) comparison of transcriptional expression levels in six different developmental stages of seeds using a custom Agilent 8x60K oligonucleotide gene expression microarray. A total of 793,875 high quality reads with an average length of 358 bp were generated and assembled into 19,841 unique full-length contigs, of which 13,705 could be annotated with GO terms. Microarray data analysis identified a total of 9,111 contigs which were differentially expressed between the six developmental stages (based on P-value of < 1e-8). The microarray-based differential expression results were validated for 192 putative genes using high-throughput quantitative real-time PCR. Results from Gene Ontology (GO) and pathway enrichment analysis showed that the upstream biosynthesis pathways were relatively active among differentially expressed sequences (DESs) during seed development, such as starch and sucrose metabolism, glycolysis/gluconeogenesis, glycerophospholipid metabolism, amino sugar and nucleotide sugar metabolism. Further, DESs related to hormone and seed development, flavonoid biosynthesis-related pathways were over-represented. A pairwise comparison was used to discover particular seed maturation dependent patterns of gene expression. Genes related to fatty acid biosynthesis were over represented in early stage, while flavonoid biosynthesis in the late stage. The data provide a useful resources and references for the seed transcriptome of J. curcas and other related species with oil producing seeds. The identified transcripts related to seed development provide a starting point for further investigation into the molecular mechanisms underlying this process.
2018-05-31 | GSE109931 | GEO
Project description:Identification of microRNAs and transcript targets in Jatropha seeds
| PRJNA218216 | ENA
Project description:Two Euphorbiaceae oil trees, Vernicia and Jatropha, were chosen to make a comparative transcriptomic study, with a focus on the differential oil accumulation process. Transcriptome sequencing was conducted with seeds at the initial- and fast- stage of oil accumulation from both.
2018-12-29 | GSE76386 | GEO
Project description:This work present a large data set related to a non-target LC-MS/MS high-resolution metabolome analysis of the leaves and roots of Jatropha curcas. Besides its high protein contents, the seeds of J. curcas are also rich in oil, so the seeds are considered a potential source of raw material to produce biodiesel and a source of food for farm animals. The full exploitation of these potentials is hampered by the presence of a highly toxic class of diterpenoids, the phorbol esters (PE), both in the oil and in the seedcake derived from the extraction of the oil. The seeds are known to possess 6 different PE, and although their structures have been known for almost 30 years, the biosynthetic pathway of each one remains undetermined. However, there are indications that the enzyme casbene synthase is responsible for the first biosynthetic step. The leaves and roots of this species are also rich in several classes of diterpenes which by themselves have pharmacological potential not yet explored.
For our analysis, we used two genotypes that are contrasting regarding the contents of phorbol esters: One genotype has a high level of phorbol esters (HPE), while the other has low levels of phorbol esters in the seeds (LPE). We were able to detect a total of 2032 features and applied multivariate statistical methods, such as PCA and heatmap with hierarchical clustering to analyze and visualize the deposition pattern of metabolites, with a focus on terpenoids. Our analysis allowed us to several new findings regarding the synthesis of PE in J. curcas, such as that only in HPE genotypes roots are found diterpenes with the tigliane skeleton. In contrast, the LPE genotype has a higher concentration and diversity of sesquiterpenes. Taking into consideration the results of our previous proteome analysis of seeds, roots, and leaves and the metabolome data presented in the manuscript, we suggest that the absence of PE in the LPE genotype may be the result of the absence of the enzymes responsible for the final biosynthetic steps, which in turn lead to the accumulation of sesquiterpenes this genotype.
| MSV000092920 | MassIVE
Project description:As a promising energy plant for biodiesel, Jatropha curcas is a tropical and subtropical shrub. Chilling is a major abiotic stress affecting the growth and development of J. curcas. In this study, we adopt the phosphoproteomic analysis, physiological measurement, ultrastructure observation to illustrate molecular mechanisms of J. curcas seedling under chilling (4 °C) stress. After chilling for 6 h, 308 significantly changed phosphoproteins were detected in J. curcas seedling without obvious physiological injury. When obvious physiological injury can be observed after chilling for 24 h, a total of 332 phosphoproteins were examined to be significantly changed, after recovery (28 °C) for 24 h, 291 phosphoproteins were varied at the phosphorylation level. The results of Gene Ontology analysis showed that phosphoproteins were mainly responsible for cellular protein modification process, transport, cellular component organization and signal transduction at the chilling and recovery periods. On the basis of protein-protein interaction network analysis, several protein kinases, such as SnRK2 (serine threonine-protein kinase srk2), MEKK1 (mitogen-activated protein kinase kinase kinase 1), EDR1, CDPK (calcium-dependent protein kinase), EIN2 (Ethylene-insensitive protein 2), EIN4, PI4K (phosphatidylinositol 4-kinase alpha 1) and 14-3-3 were possible responsible for cross-talk between ABA, Ca2+, ethylene, phosphoinositide and 14-3-3 mediated signaling pathways. We also highlighted the phosphorylation of HOS1 (E3 ubiquitin-protein ligase HOS1), APX (Cytosolic ascorbate peroxidase-1) and PIP2 (Aquaporin pip2) played vital roles in J. curcas seedling under chilling stress, and they will be valuable in further study form the molecular breeding perspective.
2019-01-09 | PXD011438 | Pride
Project description:Here, we used various quantitative proteomic strategies to establish the proteomes of roots, leaves and endosperm of two genotypes of J. curcas with contrasting levels of phorbol esters in the seeds.
2020-05-20 | PXD015103 | Pride
Project description:De novo assembly and transcriptome analysis of five major tissues of Jatropha curcas L. using GS FLX Titanium Platform of 454 pyrosequencing
| PRJNA79875 | ENA
Project description:Jatropha curcas Transcriptome or Gene expression
| PRJNA265009 | ENA