Project description:To explain the particular behavior of the mutant ΔpnpA strain, we performed a comparative transciptomic analysis of RNA isolated from a derivative strain ΔpnpA mutant versus the wild-type (WT) , after 3h and 6 h of growth in GM17 medium under semi-aerobic conditions.
Project description:To explain the particular behavior of the mutant ΔBac strain and the impact of the bacteriocin DD14 on the global regulation and gene expression in Ent. faecalis 14, we performed a comparative transciptomic analysis of RNA isolated from a derivative strain ΔBac mutant versus the wild-type (WT) , after 6 h of growth in GM17 medium under semi-aerobic conditions.
Project description:To further investigate the homeostatic response of E. faecalis to Fe exposure, we examine the whole-genome transcriptional response of wild-type (WT) exposed to non toxic Fe excess. This experiment correspond the work titled Transcriptomic response of Enterococcus faecalis to iron excess (work in preparation)
Project description:Genome-scale models represent the link between an organism's genetic information and experimentally observable biological phenotypes. They facilitate metabolic engineering and the discovery of network properties such as the identification of novel drug targets. Most commonly, metabolite consumption data is used to limit the solution space, sometimes in combination with gene expression data. However, information about gene expression only poorly correlates with the abundance of the respective proteins within the cell. As such, we developed a method to map and integrate the whole-cell proteome into genome-scale models on the example of lactic acid bacteria (LAB). To the best of our knowledge, this work represents the first effort to integrate proteome data into genome-scale models on such a scale.
