Project description:Wood in conifers is mainly composed of tracheids. Some taxa, such as Pinus, present tracheids also in the rays, but are axial tracheids which constitute the vast majority of secondary xylem. Nevertheless, radial and axial parenchyma surrounding constitutive and traumatic resin ducts is known to serve as crucial reserve storage. These reserves are mobilized in response to traumatism, insect and pathogen attacks and defoliation, allowing the synthesis of resin, healing and, in few taxa, even resprouting. However, due to the low proportion of parenchymatic cells in secondary xylem relevant genes involved in their differentiation may have been missed in studies of transcriptomics of conifer wood formation. In this study we have used Pinus canariensis as a model species, given its comparatively high proportion of axial parenchyma. We have prepared two normalized libraries from its cambial zone, covering early- and late-wood differentiation. We have de novo assembled a transcriptome, and have analyzed the transcriptional profiles during the growing season, getting a more complete picture of wood formation in conifers. SUBMITTER_CITATION: Chano, V., López de Heredia, U., Collada, C., et al. (2017). Transcriptomic analysis of juvenile wood formation during the growing season in Pinus canariensis. Holzforschung, 0(0), pp. -. Retrieved 8 Aug. 2017, from doi:10.1515/hf-2017-0014
Project description:The shoot apical meristem is responsible of seasonal length increase in plants. In woody plants transition from primary to secondary growth is also produced during seasonal apical growth. These processes are controlled by different families of transcription factors. Using a previously constructed Pinus canariensis transcriptome, we designed a genomic microarray to measure the levels of transcription during apical growth. The identification of differentially expressed genes was performed by mean of a time-course analysis.
Project description:Expression profiling by microarray hybridization Newly formed resprouts from Pinus canariensis at three different stages (R0, R1 and R2), and associated phloematic and meristematic tissue, was used for gene expression analysis against control tissues, sampled at the same dates from remote parts of the plant, in order to check transcriptomical variations of an unusual trait in conifers.
Project description:Gene expression analysis in response to mechanical injuries in Pinus canariensis, known for a great healing ability. Traumatic xylem formed in the margins of the wounds were compared to control tissues, sampled from remote parts of the plant, in order to check transcriptomical variations in three dates during the response: H1, H2 and H3 for 7, 78 and 92 days after wounding.
