Project description:Array analysis of total RNA from broncho-alveolar lavage (BAL) from mice infected with influenza virus (PR8) and/or Staphylococcus aureus (Newman)
Project description:In this study broncho-alveolar lavage fluid (BALF) from mice infected with influenza A virus, PR8 (280 PFU) was analyzed for lipidomic host response in comparison with the mock infected animals. Time Points: 6, 9, 11, 13 and 19 days post infection, done in 8-10 replicates.)
Project description:In this study broncho-alveolar lavage fluid (BALF) from mice infected with influenza A virus, PR8 (200 PFU) or X31 (200,000 PFU) was analyzed for lipidomic host response in comparison with the mock infected animals. Time Points: 3, 5, 6, 7, 9, 10 and 13 days post infection.)
Project description:In this study broncho-alveolar lavage fluid (BALF) from mice infected with influenza A virus, PR8 (200 PFU and 200,000 PFU), X31 (2000,000 PFU) was analyzed for lipidomic host response in comparison with the mock infected animals. Time Points: 3, 5, 7, 9, 11, 13, 19 days post infection, done in 8-10 replicates.)
Project description:This data set was downloaded from MetaboLights (http://www.ebi.ac.uk/metabolights/) accession number MTBLS33 Abstract:In this study broncho-alveolar lavage fluid (BALF) from mice infected with influenza A virus, PR8 (280 PFU) was analyzed for lipidomic host response in comparison with the mock infected animals. Time Points: 3, 6, 9 and 11 days post infection.
Project description:To gain insights into the early immune dynamics of transcriptional changes during SARS-CoV-2 infection in airways, we performed longitudinal scRNA-Seq of the broncho-alveolar lavage (BAL) cells isolated from SARS-CoV-2 infected rhesus macaques. We found early induction of innate type-1 interferon responses with accumulation of a distinct macrophage population that possesses an interferon-driven innate anti-viral gene signature early during infection.
Project description:This data set was downloaded from MetaboLights (http://www.ebi.ac.uk/metabolights/) accession number MTBLS32 Abstract:In this study broncho-alveolar lavage fluid (BALF) from mice infected with influenza A virus, PR8 (280 PFU) was analyzed for lipidomic host response in comparison with the mock infected animals. Time Points: 6, 9, 11, 13 and 19 days post infection, done in 8-10 replicates.)
Project description:This data set was downloaded from MetaboLights (http://www.ebi.ac.uk/metabolights/) accession number MTBLS34 Abstract:In this study broncho-alveolar lavage fluid (BALF) from mice infected with influenza A virus, PR8 (200 PFU) or X31 (200,000 PFU) was analyzed for lipidomic host response in comparison with the mock infected animals. Time Points: 3, 5, 6, 7, 9, 10 and 13 days post infection.)
Project description:We have defined naïve, central memory, effector memory and terminally differentiated porcine CD8 T cells, using CD45RA and CCR7 mAbs. We analyzed the phenotype of CD8 T cells specific for three influenza nucleoprotein (NP) epitopes using tetramers after influenza infection or immunization in lymphoid and respiratory tissues. The hierarchy of response to the three epitopes changes during the response in different tissues. Most NP-specific CD8 T cells in broncho-alveolar lavage (BAL) and lung are tissue resident memory cells (TRM), that express CD69 and have an effector memory or terminally differentiated phenotype. NP-specific cells isolated from BAL express genes characteristic of TRM, but gene expression differs at 7, 21 and 63 days post infection. The frequency of NP-specific cells declines over 63 days in all tissues but is best maintained in BAL. The pig is a powerful model for understanding how best to induce and harness local immunity.
