Schizosaccharomyces pombe
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA326135 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Acetylation and dimethylation of lysine 9 on histone H3 are prominent marks of euchromatin and heterochromatin, respectively. Moreover, histone acetylation has been linked to lipid metabolism. Cut6, the acetyl-CoA carboxylase, is one of direct targets of Cbf11 which we have previously identified as a regulator of lipid metabolism. Here we have performed ChIP-seq of H3K9ac, H3K9me2 and H3 in Pcut6MUT strain (promoter mutant of cut6 with abolished Cbf11 binding) of Schizosaccharomyces pombe in three biological replicates. Strain genotypes: cut6 promoter mutant MP636 (h- Pcut6MUT).
2023-05-24 | E-MTAB-11082 | biostudies-arrayexpress
Project description:Heterochromatic DNA domains play important roles in the regulation of gene expression and maintenance of genome stability by silencing repetitive DNA elements and transposons. In organisms ranging from fission yeast to mammals, heterochromatin assembly at DNA repeats involves the activity of small noncoding RNAs (sRNAs) associated with the RNA interference (RNAi) pathway. Typically, sRNAs, originating from long noncoding RNAs transcribed from DNA repeats, guide Argonaute-containing effector complexes to complementary nascent RNAs to initiate histone H3 lysine 9 di- and tri-methylation (H3K9me2 and H3K9me3, respectively) and heterochromatin formation. H3K9me is in turn required for recruitment of RNAi to chromatin and promotes sRNA generation. However, how heterochromatin formation, which silences transcription, can proceed by a co-transcriptional mechanism that also promotes sRNA generation remains paradoxical. Here, using Clr4, the fission yeast homolog of mammalian SUV39H H3K9 methyltransferases, we designed active site mutations, which allow H3K9me2 catalysis but block the transition to H3K9me3. We show that H3K9me2 defines a functionally distinct heterochromatin state that is sufficient for RNAi-dependent co-transcriptional gene silencing (CTGS). Unlike H3K9me3 domains, which are transcriptionally silent, H3K9me2 domains are transcriptionally active, contain modifications associated with euchromatic transcription, and couple RNAi-mediated transcript degradation to the establishment of H3K9me domains. The two H3K9me states recruit reader proteins with different efficiencies, explaining their different downstream silencing functions. Furthermore, transition from H3K9me2 to H3K9me3 is required for RNAi-independent epigenetic inheritance of H3K9me domains. Our findings demonstrate that H3K9me2 and H3K9me3 define functionally distinct heterochromatin states and uncover a mechanism for formation of transcriptionally permissive heterochromatin that is compatible with its broadly conserved role in RNAi-mediated genome defense.
2017-07-27 | GSE83495 | GEO
Project description:Fission yeast Jmj2 reverses histone H3 lysine 4 tri-methylation
| PRJNA101077 | ENA
Project description:Localization of histone H3 and histone H3 lysine 4 trimethylation at the start of pre-meiotic S phase
| PRJNA233625 | ENA
Project description:In the fission yeast Schizosaccharomyces pombe, the RNA interference (RNAi) pathway is required to generate small interfering RNAs (siRNAs) that mediate heterochromatic silencing of centromeric repeats. Here we demonstrate that RNAi also functions to repress genomic elements other than constitutive heterochromatin. Using DamID (DNA adenine methyltransferase identification) we show that Dcr1 and Rdp1 physically associate with some euchromatic genes, non-coding RNA (ncRNA) genes, and retrotransposon long terminal repeats (LTRs), and that this association is independent of the Clr4 histone methyltransferase. Physical association of RNAi with chromatin is sufficient to trigger a silencing response but not to assemble heterochromatin. The mode of silencing at the newly identified RNAi targets is consistent with a co-transcriptional gene silencing model as proposed earlier and functions with trace amounts of siRNAs. We anticipate that similar mechanisms could also be operational in other eukaryotes.
2010-09-25 | GSE24360 | GEO
Project description:Acetylation and dimethylation of lysine 9 on histone H3 are prominent marks of euchromatin and heterochromatin, respectively. Moreover, histone acetylation has been linked to lipid metabolism. We have previously identified the transcription factor Cbf11 as a regulator of lipid metabolism and genome integrity in the fission yeast. Cut6, the acetyl-CoA carboxylase, is one of direct targets of Cbf11. To link the role of Cbf11 in lipid metabolism and chromatin regulation we have performed ChIP-seq of H3K9ac, H3K9me2 and H3 in WT and cbf11KO strains of Schizosaccharomyces pombe in three biological replicates. Strain genotypes: wild type JB32 (h+s); cbf11 knock-out MP44 (h+ cbf11::natR).
2021-11-03 | E-MTAB-11081 | biostudies-arrayexpress
Project description:Mediator directs co-transcriptional heterochromatin assembly by RNAi-dependent and -independent pathways.
| PRJNA186678 | ENA
Project description:The Chromatin-Remodeling Factor FACT Contributes to Centromeric Heterochromatin Independently of RNAi
| PRJNA98201 | ENA
Project description:Histone H2A.Z cooperates with RNAi and heterochromatin factors to suppress antisense RNAs
| PRJNA119129 | ENA
Project description:Genome wide map of heterochromatin state in fission yeast Schizosaccharomyces pombe
| PRJNA183637 | ENA