Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Real-time quantitative PCR analysis of murine splenic chemokines and cytokines

ABSTRACT: Real-time quantitative PCR analysis of murine splenic chemokines and cytokines

PROVIDER: PRJNA328471 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Real-time quantitative PCR analysis of murine splenic chemokines and cytokines [chemokines & receptors assay]

Project description:C57BL/6 mice were subjected to sham surgery or orthotopic Hepa1-6 hepatic tumor inoculation. Spleen tissues from mice subjected to sham surgery (control mice), mice bearing a 2-week orthotopic hepatoma, and mice bearing a 4-week othotopic hepatoma were collected. Total RNA was extracted from the spleen tissues,and we used the RT² Profiler PCR array-mouse chemokines & receptors (SABioscience) array panel to quantitate gene expression of chemokine and receptor genes from the spleens.

2016-07-12 | GSE84246 | GEO

Real-time quantitative PCR analysis of murine splenic chemokines and cytokines [common cytokines assay]

Project description:C57BL/6 mice were subjected to sham surgery or orthotopic Hepa1-6 hepatic tumor inoculation. Spleen tissues from mice subjected to sham surgery (control mice), mice bearing a 2-week orthotopic hepatoma, and mice bearing a 4-week othotopic hepatoma were collected. Total RNA was extracted from the spleen tissues, and we used the RT² Profiler PCR array-mouse common cytokines (SABioscience) array panels to quantitate gene expression of cytokine genes from the spleens.

2016-07-12 | GSE84247 | GEO

Cytokines & Chemokines PCR Array on human DC

Project description:Stimulation of human DC with LPS, VSL#3, or a combination of both resulted in their maturation, evident from enhanced expression of activation markers on the cell-surface, as well as the induction of various chemokines and cytokines.

2014-10-23 | GSE62583 | GEO

Real-time quantitative PCR analysis of cytokines and chemokines in C57BL/6 mouse liver ischemic-reperfusion injury (IRI) model.

Project description:We assessed mouse cytokines and chemokines gene expression in fatty liver ischemic-reperfusion injury (IRI). C57BL/6 mice were fed with a normal chow diet (ND, 4.09 kcal/gram,13.4% kJ/fat) or a high-fat diet (HFD, 5.10 kcal/gram, 60% kJ/fat) and further grouped to treat with orally administered N-acetylcysteine (NAC, 10g/L). To induce liver ischemic-reperfusion injury in mice, an atraumatic micro clip was placed across the hepatic hilus, which interrupted the blood supply to the left and median lobes of the liver for 45-minutes partial warm ischemia time. After 24 hours of liver IRI, the affected left lobe of the liver was harvested and stored in Allprotect Tissue Reagent (Qiagen). We used Qiagen RT² Profiler™ PCR Array for mouse cytokines & chemokines to distinguish immunologically related gene signatures specific to the liver IRI in mice and characterize the effect of NAC treatment in HFD mice.

2022-07-30 | GSE209733 | GEO

LPS induced Cytokines & Chemokines changes in E9.5 pregnant mouse uteri

Project description:Changes of Cytokines and chemokines profiles were sucessfully determined between mouse non-treated vs LPS treated pregnant uteri using Qiagen PCR array.

2015-01-01 | GSE63246 | GEO

Real-time quantitative PCR analysis of murine splenic chemokines and cytokines

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-07-12 | GSE84248 | GEO

Mus musculus

Project description:Real-time quantitative PCR analysis of murine splenic chemokines and cytokines [chemokines & receptors assay]

| PRJNA328476 | ENA

Mus musculus

Project description:Real-time quantitative PCR analysis of murine splenic chemokines and cytokines [common cytokines assay]

| PRJNA328475 | ENA

LPS induced Cytokines & Chemokines changes in E9.5 pregnant mouse uteri

Project description:Changes of Cytokines and chemokines profiles were sucessfully determined between mouse non-treated vs LPS treated pregnant uteri using Qiagen PCR array. Pregnant mouse (FVB strain E8.5-9.5) were treated with LPS(2.5ug/mouse) or saline as control. 8h- or 16-h later, uteri tissues (N=3) were collected.

2015-01-01 | E-GEOD-63246 | biostudies-arrayexpress

Real-time quantitative PCR analysis of human low-density (LDNs) and normal-density neutrophils (NDNs)

Project description:NDNs and LDNs were isolated by density gradient centrifugation of PBMCs from 8 patients affected by tubercolosis (TB). In detail, RNA was extracted from NDNs and LDNs by Maxwell® RSC miRNA Tissue kit (Promega, Madison, USA), following manufacturer’s instructions. RNA concentration and quality was measured using NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific). Total RNA (600 ng) was reverse transcribed using the RT2 First Strand Kit (SABiosciences, Qiagen, UK). 84 human cytokines and chemokines were tested using the RT2 Profiler PCR Array Human Innate & Adaptive Immune Responses array (Qiagen). A semiquantitative real-time RT-PCR was performed using a real-time PCR detection system (QuantStudio 7 Flex Real-Time PCR System, Thermo Fisher Scientific) with a two-step thermal cycling: 95 °C for 10 min, followed by 40 cycles (95 °C for 15 sec, 60 °C for 1 min). Data were analysed using the RT² Profiler PCR Array Qiagen data analysis software, and ACTB and B2M as housekeeping genes.

2019-11-20 | GSE138948 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data